Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to enhance neurite outgrowth of E16-E18 rat embryonic cortical neurons. Able to significantly enhance neurite outgrowth when immobilized at 1.9-15 μg/mL on a nitrocellulose-coated microplate.
Source
Mouse myeloma cell line, NS0-derived Gln19-Pro910, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
AnalysisNo results obtained: Gln19 predicted, sequencing might be blocked
Structure / Form
Noncovalently-linked homodimer
Predicted Molecular Mass
99.8 kDa (monomer)
SDS-PAGE
115-125 kDa, reducing conditions
7587-MD |
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Formulation Lyophilized from a 0.2 μm filtered solution in PBS. | ||
Reconstitution Reconstitute at 300 μg/mL in PBS. | ||
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | ||
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Background: MDGA1
MDGA1 (MAM domain containing glycosylphosphatidylinositol anchor 1) is a 135-140 kDa glycoprotein within the IgCAM superfamily of adhesion molecules (1-3). MDGA1 is thought to mediate neuronal migration, neurite outgrowth, and cell-cell adhesion within the central and peripheral nervous system (1, 4-8). Mouse MDGA1 precursor is a 940 amino acid (aa) protein that produces an 899 aa mature protein with six Ig-like domains, a fibronectin type III domain (aa 622-724), a MAM domain (aa 736-903), and a GPI anchor (aa 917) (1-3). Mature mouse MDGA1 shares 95%, 97%, and 94% aa sequence identity with mature human, rat, and canine MDGA1, respectively. Mouse MDGA1 also shares 52% aa sequence identity with mouse MDGA2, and may also share some functional redundancy (1, 4). MDGA1 is mainly expressed on restricted populations of neurons in the central and peripheral nervous system, such as embryonic neurons destined for cortical layers 2/3, migrating basilar pontine neurons and D1 interneurons of the spinal cord (1, 5-7). Deletion or down‑regulation of mouse MDGA1 slows radial migration of neurons, indicating a role for MDGA1 in radial migration of cortical neurons (4, 5). MAM and Ig-like domains are involved in heterophilic and homophilic adhesion (7, 8). MDGA1 expression is also reported on primary cells and cell lines from leukemias, lymphomas, and tumors of the lung, colon, uterus, stomach and breast (3).
References:
Litwack, E.D. et al. (2004) Mol. Cell. Neurosci. 25:263.
Diaz-Lopez, A. et al. (2005) Exp. Cell Res. 307:91.
De Juan, C. et al. (2002) Oncogene 21:3089.
Ishikawa, T. et al. (2011) Dev. Dyn. 240:96.
Takeuchi, A. and D.D.M. O'Leary (2006) J. Neurosci. 26:4460.
Takeuchi, A. et al. (2007) Cereb Cortex. 17:1531.
Fujimura, Y. et al. (2006) Brain Res. 1101:12.
Diaz-Lopez, A. et al. (2011) Cancer Microenviron. 4:23.
Long Name:
MAM Domain Containing Glycosylphosphatidylinositol Anchor 1
Entrez Gene IDs:
266727 (Human); 74762 (Mouse)
Alternate Names:
FLJ45018; GPIM; MAM domain containing glycosylphosphatidylinositol anchor 1; MAMDC3; MDGA1
