Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by the ability of the immobilized protein to support the adhesion of EL‑4 mouse lymphoblast cells. The ED 50 for this effect is 1-5 μg/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived
Mouse Mill2
(Ser30-His298)
Accession # NP_715641IEGRMDP Mouse IgG2A
(Glu98-Lys330)N-terminus C-terminus Accession #
N-terminal Sequence
AnalysisSer30
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
57.6 kDa (monomer)
SDS-PAGE
60-66 kDa, reducing conditions
7657-ML |
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Formulation Lyophilized from a 0.2 μm filtered solution in PBS. | ||
Reconstitution Reconstitute at 250 μg/mL in PBS. | ||
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | ||
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Background: Mill2
Mill2 (MHC class I‑like located near the leukocyte receptor complex‑2) is an ~41 kDa glycosyl‑phosphatidylinositol (GPI)‑linked glycoprotein that is a member of the non‑classical MHC class I‑like family (1‑3). Mouse Mill2 cDNA encodes 340 amino acids (aa) that include a signal sequence, an MHC I domain containing alpha 1, alpha 2 and alpha 3 regions, and a GPI anchor sequence (1). A long form contains 15 aa inserted near the N‑terminus. Mature mouse Mill2 (short form) shares 74% aa sequence identity with rat Mill2 and approximately 61% aa identity with mouse Mill1. Human orthologs of Mill proteins have not been identified, although similarities in structure and sequence with human HFE and MICA/B have been noted (1, 4, 5). Mill2 is expressed on proliferating thymocytes, smooth muscle cells and fibroblasts in skin, bladder, uterus and colonic muscularis (5). Low expression is detected in many tumor cell lines (5). Mill proteins bind beta 2‑microglobulin (2). While Mill1 requires beta 2‑microglobulin for surface expression, the short form of Mill2 does not, and surface expression of the long form is higher in its presence (5). Mill proteins are unlikely to present peptides and do not bind to NKG2D (2, 5). Recombinant Mill2 has, however, shown pH‑dependent binding to TfR/CD71‑expressing immature cells in the B lymphocyte and erythrocyte lineages, as well as activated, proliferating splenic T and B cells and some cell lines (5).
References:
Kasahara, M. et al. (2002) Proc. Natl. Acad. Sci. USA 99:13687.
Kajikawa, M. et al. (2006) J. Immunol. 177:3108.
Martinez-Naves, E. et al. (2011) FEBS Lett. 585:3478.
Watanabe, Y. et al. (2004) Eur. J. Immunol. 34:1597.
Rabinovich, B.A. et al. (2008) Immunol. Cell Biol. 86:489.
Long Name:
MHC I-like Leukocyte 2
Entrez Gene IDs:
243864 (Mouse)
Alternate Names:
Mill2
