Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit Flt-3 Ligand-induced proliferation of BaF3 mouse pro‑B cells transfected with Flt-3. The ED 50 for this effect is 0.05-0.25 µg/mL in the presence of recombinant mouse Flt-3 Ligand at 10 ng/mL.
Source
Mouse myeloma cell line, NS0-derived
Mouse Flt-3
(Asn28-Ser544)
Accession # Q00342IEGRMD Human IgG1
(Pro100-Lys330)6-His tag N-terminus C-terminus Accession #
N-terminal Sequence
AnalysisAsn28
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
85 kDa (monomer)
SDS-PAGE
116 kDa, reducing conditions
768-F3 |
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Formulation Lyophilized from a 0.2 μm filtered solution in PBS. | ||
Reconstitution Reconstitute at 100 μg/mL in sterile PBS. | ||
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | ||
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Background: Flt-3/Flk-2
The Flt-3 (fms-like tyrosine kinase) receptor, also named Flk-2 (fetal liver kinase) and Stk-1 (stem cell tyrosine kinase), is a member of the class III subfamily of receptor tyrosine kinases. This familay includes KIT, the receptor for SCF, and C-FMS, the receptor for M-CSF. The extracellular region of these receptors contains five immunoglobulin-like domains and the intracellular region contains a split kinase domain. Mouse Flt-3 cDNA encodes a 992 amino acid (aa) residue type I membrane protein with a 27 aa residue signal peptide, a 517 aa extracellular domain with 10 potential N-linked glycosylation sites, a 20 aa residue transmembrane domain and a 428 aa residue cytoplasmic domain. Mouse Flt-3 shares 85% amino acid sequence identity with human Flt-3. Flt-3 expression has been detected in various tissues, including placenta, gonads, and tissues of nervous and hematopoietic origin. Among hematopoietic cells, the expression of Flt-3 was found to be restricted to the highly enriched stem/progenitor cell populations. The ligand for Flt-3 (FL) has been identified to be a transmembrane protein with structural homology to M-CSF and SCF. Recombinant soluble Flt-3/Fc chimeric protein has been shown to bind FL with high affinity and is a potent FL antagonist.
References:
Rosnet, O. et al. (1996) Acta. Haemato. 95:218.
Drexler, H.G. (1996) Leukemia 10:588.
Long Name:
fms-like Tyrosine Kinase 3
Entrez Gene IDs:
2322 (Human); 14255 (Mouse)
Alternate Names:
CD135 antigen; CD135; EC 2.7.10; fetal liver kinase 2; FL cytokine receptor; FLK2; Flk-2; FLT3 receptor tyrosine kinase; Flt3; Flt-3; Fms-like tyrosine kinase 3; fms-related tyrosine kinase 3; growth factor receptor tyrosine kinase type III; Stem cell tyrosine kinase 1; STK-1; STK1EC 2.7.10.1; Tyrosine-protein kinase receptor FLT3
