Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by the ability of the immobilized protein to support the adhesion of BUD‑8 human fibroblast cells. When 3 x 10 4 cells are added to Recombinant Mouse Desmoglein‑2 Fc Chimera coated plates (10 μg/mL, 100 μL/well), approximately 30%‑60% will adhere after 1 hour at 37 °C.
Optimal dilutions should be determinded by each laboratory for each application.Source
Mouse myeloma cell line, NS0-derived
Mouse Desmoglein-2
(Leu29-Ala618)
Accession # O55111IEGRMDP Mouse IgG2A
(Gln98-Lys330)N-terminus C-terminus Accession #
N-terminal Sequence
AnalysisLeu29 & Ala55
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
92.9 kDa (monomer)
SDS-PAGE
100-120 kDa, reducing conditions
7699-DM |
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Formulation Lyophilized from a 0.2 μm filtered solution in PBS. | ||
Reconstitution Reconstitute at 400 μg/mL in PBS. | ||
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | ||
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Background: Desmoglein-2
Desmoglein-2 (DSG2) is a 160 kDa transmembrane glycoprotein in the cadherin family of calcium dependent adhesion molecules. It is a major protein component of desmosomal contacts between epithelial cells (1, 2). Mature mouse Desmoglein‑2 consists of a 564 amino acid (aa) extracellular domain (ECD) with four cadherin domains, a 21 aa transmembrane segment, and a 483 aa cytoplasmic domain with six desmoglein repeats (3, 4). Within the ECD, mouse Desmoglein‑2 shares 81% and 93% aa sequence identity with human and rat Desmoglein‑2, respectively. A 60 kDa ECD fragment can be proteolytically shed, and this is enhanced by EGF R activation (5‑7). The cytoplasmic domain is cleaved during apoptosis, leaving the transmembrane segment and ECD as a cell‑associated fragment (8). Desmoglein‑2 is widely expressed in epithelia and is required for maintenance of the epithelial barrier (1, 2, 9). It can be either up- or down‑regulated in a variety of carcinomas during tumor progression (10). Desmoglein‑2 also functions as a cell adhesion receptor for several adenovirus serotypes (11). This binding disrupts intercellular junctions between epithelial cells and promotes epithelial‑mesenchymal transition (EMT) (11). Mutations in Desmoglein‑2 cause cardiac dysfunction typical of arrythmogenic right ventricular cardiomyopathy (ARVC) (12).
References:
Brooke, M.A. et al. (2012) J. Pathol. 226:158.
Thomason, H.A. et al. (2010) Biochem. J. 429:419.
Mahoney, M.G. et al. (2002) Exp. Dermatol. 11:115.
Whittock, N.V. (2003) J. Invest. Dermatol. 120:970.
Bech-Serra, J.J. et al. (2006) Mol. Cell. Biol. 26:5086.
Klessner, J.L. et al. (2009) Mol. Biol. Cell 20:328.
Ramani, V.C. et al. (2008) BMC Cancer 8:373.
Nava, P. et al. (2007) Mol. Biol. Cell 18:4565.
Schlegel, N. et al. (2010) Am. J. Physiol. Gastrointest. Liver Physiol. 298:G774.
Dusek, R.L. and L.D. Attardi (2011) Nat. Rev. Cancer 11:317.
Wang, H. et al. (2011) Nat. Med. 17:96.
Pilichou, K. et al. (2009) J. Exp. Med. 206:1787.
Entrez Gene IDs:
1829 (Human); 13511 (Mouse)
Alternate Names:
ARVC10; ARVD10; Cadherin family member 5; CDHF5; CDHF5MGC117034; CMD1BB; desmoglein 2; Desmoglein2; Desmoglein-2; DSG2; HDGC; MGC117036; MGC117037
