Recombinant Mouse Integrin alpha 11 beta 1 Protein, CF 50 UG

• Purity

  >95%, by SDS-PAGE under reducing conditions and visualized by silver stain

• Endotoxin Level

  <0.10 EU per 1 μg of the protein by the LAL method.  

• Activity

  Measured by its binding ability in a functional ELISA. When Collagen I is coated at 10 μg/mL, Recombinant Mouse Integrin alpha 11 beta 1 binds with an apparent K    _D <20 nM.  

• Source

  Chinese Hamster Ovary cell line, CHO-derived

  Mouse Integrin alpha 11 (Phe23-Pro1141) Accession # P61622	HP	GGGSGGGS	Acidic Tail	HHHHHH
  Mouse Integrin beta 1 (Gln21-Asp728) Accession # P09055	HP	GGGSGGGS	Basic Tail
  N-terminus			C-terminus

• Accession #

• N-terminal Sequence    
  Analysis

  Phe23 ( alpha 11 subunit) & Gln21 predicted: No results obtained, sequencing might be blocked ( beta 1 subunit)

• Structure / Form

  Noncovalently-linked heterodimer

• Predicted Molecular Mass

  134 kDa ( alpha 11 subunit) & 86.5 kDa ( beta 1 subunit)

• SDS-PAGE

  115-135 kDa & 155-175 kDa, reducing conditions

Background: Integrin alpha 11 beta 1

Integrin alpha 11 beta 1 is a noncovalent heterodimeric collagen receptor that contains a 150 kDa alpha 11 subunit complexed to a 130 kDa beta 1/CD29 subunit.  alpha 11 beta 1 is the only known alpha 11-containing integrin. By contrast, at least twelve alpha -integrins share the beta 1 subunit (1-5). alpha 11 beta 1 is expressed on fibroblasts from cornea, skin and periodontal ligament (3-9). It is also expressed on mesenchymal stem cells and is considered a marker for non-muscle mesenchymal tissue (3, 8-10). Expression of alpha 11 beta 1 is stimulated by TGF-beta 1, activin A, and type I interferons, and down‑regulated by FGF-2 (6, 9-11). The alpha 11 extracellular domain (ECD) contains a vWFA domain which includes the ligand binding site (2-5). The beta 1 ECD also contains a vWFA domain, which participates in binding. Each subunit has a transmembrane sequence and a short cytoplasmic tail. The dimer assumes a folded configuration at "rest". Divalent cations and intracellular (inside-out) signaling convert it to an active, extended and open conformation (1, 2). The 1119 amino acid (aa) mouse alpha 11 ECD shares 90% and 95% aa sequence identity with rat and human alpha 11, respectively, while the 708 aa mouse beta 1 ECD shares 98% aa identity with rat and 93-94% aa identity with human, bovine, porcine, ovine, canine and feline beta 1. I domain-containing beta 1 integrins alpha 1 beta 1, alpha 2 beta 1, alpha 10 beta 1 and alpha 11 beta 1 all bind collagens (3-5). alpha 11 beta 1 preferentially binds interstitial collagens such as type I (5, 8, 12). It can contract collagen matrices and influence inflammatory edema (3, 13). Depending on context, alpha 11 beta 1 can support or inhibit cell migration on collagen (7, 8). It is frequently up‑regulated in the stroma of lung adenocarcinomas and enhances tumorigenicity (14, 15). alpha 11 beta 1 is also involved in myofibroblast differentiation in human corneal fibroblasts and human cardiac fibroblasts (9, 16).

• References:

1. Takada, Y. et al. (2007) Genome Biol. 8:215.

2. Luo, B-H. et al. (2007) Annu. Rev. Immunol. 25:619.

3. Popova, S.N. et al. (2007) Acta Physiol. 190:179.

4. Lehnert, K. et al. (1999) Genomics 60:179.

5. Velling, T. et al. (1999) J. Biol. Chem. 274:25735.

6. Gouttenoire, J. et al. (2010) Eur. J. Cell Biol. 89:307.

7. Popova, S.N. et al. (2004) Dev. Biol. 270:427.

8. Tiger, C-F. et al. (2001) Dev. Biol. 237:116.

9. Carrecedo, S. et al. (2010) J. Biol. Chem. 285:10434.

10. Varas, L. et al. (2007) Stem Cells Dev. 16:965.

11. Coelho, L.F.L. et al. (2006) Cytokine 33:352.

12. Zhang, W-M. et al. (2003) J. Biol. Chem. 278:7270.

13. Svendsen, O.S. et al. (2009) Arterioscler. Thromb. Vasc. Biol. 29:1864.

14. Wang, K-K. et al. (2002) Oncogene 21:7598.

15. Zhu, C-Q. et al. (2007) Proc. Natl. Acad. Sci. USA 104:11754.

16. Talior-Volodarsky, I. et al. (2012) Cardiovasc. Res. 96:265.

• Alternate Names:

  Integrin alpha 11 beta 1