Purity
>95%, by SDS-PAGE with silver staining
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by the ability of the immobilized protein to support the adhesion of HEK293 human embryonic kidney cells. The ED 50 for this effect is 0.15 - 0.9 µg/mL.
Source
Mouse myeloma cell line, NS0-derived Leu18-Ala716, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
AnalysisNo results obtained, Leu18 predicted. Protein identity confirmed by MS analysis of tryptic fragments.
Predicted Molecular Mass
77 kDa (monomer)
SDS-PAGE
85-105 kDa, reducing conditions
7926-CA |
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Formulation Lyophilized from a 0.2 μm filtered solution in PBS. | ||
Reconstitution Reconstitute at 500 μg/mL in sterile PBS. | ||
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | ||
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Background: Protocadherin-12
Protocadherin-12 (PCDH12; also called VE-Cadherin-2) is an approximately 150 kDa glycoprotein belonging to the δ2 subgroup of non-clustered protocadherins. These are type I transmembrane proteins that exhibit calcium-dependent but relatively weak homophilic adhesion (1-4). Like other δ2 protocadherins, mature Protocadherin-12 contains six cadherin domains in its extracellular domain (ECD), a transmembrane sequence, and a cytoplasmic domain (1). Within the ECD, mouse Protocadherin-12 shares 95% and 82% amino acid sequence identity with rat and human Protocadherin-12, respectively. Protocadherin-12 is expressed in mouse endothelial, glycogen-rich trophoblast, and mesangial cells where it clusters at intercellular junctions (1, 2). It may be important during placental development, as placentas from Protocadherin-12-deficient mice display altered morphogenesis and transcription profiles (3). In humans, the ECD of Protocadherin-12 can be shed via proteolytic cleavage (5). It circulates at increased levels in sera from pregnant women exhibiting pre-eclampsia compared to women with nonpathological gestations (5).
References:
Telo’, P. et al. (1998) J. Biol. Chem. 273:17565.
Rampon, C. et al. (2005) Exp. Cell Res. 302:48.
Rampon, C. et al. (2008) Physiol. Genomics 34:193.
Kim, S.Y. et al. (2011) Cell Adh. Migr. 5:97.
Bouillot, S. et al. (2011) J. Biol. Chem. 286:15195.
Entrez Gene IDs:
51294 (Human); 53601 (Mouse)
Alternate Names:
PCDH12; protocadherin 12; Protocadherin12; Protocadherin-12; Vascular cadherin-2; vascular endothelial cadherin 2; Vascular endothelial cadherin-2; VECAD2; VE-cad-2; VE-cadherin 2; VE-cadherin-2; VE-cadherin-2protocadherin-12
