Purity
>95%, by SDS-PAGE with silver staining
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Mouse Mesothelin is immobilized at 2 μg/mL (100 μL/well), the concentration of Recombinant Human CA125/MUC16 (Catalog # ) that produces 50% optimal binding response is approximately 0.4-2.4 ng/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived Asp298-Ser600, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
AnalysisAsp298
Predicted Molecular Mass
35 kDa
SDS-PAGE
38-55 kDa, reducing conditions
8604-MS |
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Formulation Lyophilized from a 0.2 μm filtered solution in PBS with trehalose. | ||
Reconstitution Reconstitute at 250 μg/mL in PBS. | ||
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | ||
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Background: Mesothelin
Mesothelin, also known as CAK1 and ERC, is derived from a 70 kDa precursor that also includes Megakaryocyte Potentiating Factor (MPF) (1-3). The 70 kDa precursor is expressed on the cell surface where it is cleaved at a dibasic proteolytic site to release the 32 kDa glycosylated MPF (3, 4). MPF is a cytokine that potentiates IL-3 induced megakaryocyte colony formation (2, 5). The term Mesothelin refers to the 40 kDa glycosylated protein which remains attached to the cell surface via a GPI linkage. Mesothelin is normally expressed on mesothelial cells in the pleura, pericardium, and peritoneum as well as in the developing and postnatal pancreas (1, 6). It is up-regulated in mesotheliomas and a range of carcinomas and adenomas (7-10). Mesothelin promotes tumor cell proliferation, migration, anchorage-independent growth, and tumor progression, in part through the up-regulation of MMP-7 (9, 11, 12). It is co-expressed with the tumor antigen CA125/MUC16 on advanced ovarian adenocarcinomas and interacts with this molecule to support cell adhesion (13). A soluble form of Mesothelin, with its GPI anchor intact, is released from tumor cells and binds to MMR/CD206 on macrophages (10, 14-16).
References:
Hassan, R. et al. (2004) Clin. Cancer Res. 10:3937.
Kojima, T. et al. (1995) J. Biol. Chem. 270:21984.
Chang, K. and I. Pastan (1996) Proc. Natl. Acad. Sci. 93:136.
Onda, M. et al. (2006) Clin. Cancer Res. 12:4225.
Yamaguchi, N. et al. (1994) J. Biol. Chem. 269:805.
Hou, L.-Q. et al. (2008) Develop. Growth Differ. 50:531.
Ordonez, N.G. (2003) Mod. Pathol. 16:192.
Argani, P. et al. (2001) Clin. Cancer Res. 7:3862.
Li, M. et al. (2008) Mol. Cancer Ther. 7:286.
Scholler, N. et al. (1999) Proc. Natl. Acad. Sci. 96:11531.
Uehara, N. et al. (2008) Mol. Cancer Res. 6:186.
Chang, M.-C. et al. (2012) Biochem. J. 442:293.
Rump, A. et al. (2004) J. Biol. Chem. 279:9190.
Ho, M. and M.O. Lively (2006) Cancer Epidemiol. Biomarkers Prev. 15:1751.
Robinson, B.W.S. et al. (2003) Lancet 362:1612.
Dangaj, D. et al. (2011) PLoS ONE 6:e28386.
Entrez Gene IDs:
10232 (Human); 56047 (Mouse); 60333 (Rat)
Alternate Names:
CAK1 antigen; CAK1; megakaryocyte potentiating factor; Mesothelin; MPF; MPFSMRP; MSLN; Pre-pro-megakaryocyte-potentiating factor; SMR; soluble MPF mesothelin related protein
