Purity
>90%, by SDS-PAGE with silver staining
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by the ability of the immobilized protein to support the adhesion of SVEC4‑10 mouse vascular endothelial cells. The ED 50 for this effect is 0.7-3.5 μg/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived Gln25-Phe440, with an N-terminal HA Tag
Accession #
N-terminal Sequence
AnalysisTyr
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
47 kDa
SDS-PAGE
53-63 kDa, reducing conditions
8606-FB |
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Formulation Lyophilized from a 0.2 μm filtered solution in PBS. | ||
Reconstitution Reconstitute at 500 μg/mL in PBS. | ||
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. | ||
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Background: Fibulin 7
Fibulin 7, also known as TM14, is an approximately 46 kDa member of the fibulin family of proteins (1, 2). Structurally, Fibulin 7 contains an N-terminal Sushi domain, 3 central tandem EGF modules, and a C-terminal domain homologous to the fibulin module (2). Mouse Fibulin 7 mRNA is highly expressed in teeth, with weaker expression in brain, kidneys, muscles, and bones (2). Mouse Fibulin 7 shares 86% amino acid sequence identity with human Fibulin 7. Fibulin 7 interacts with several extracellular molecules, including Fibronectin, DSPP, and Fibulin 1 (2). A C-terminal fragment of Fibulin 7 was able to bind Human Umbilical Vein Endothelial Cells (HUVECs) and inhibit HUVEC tube formation and vessel sprouting in aortic ring assays, suggesting that Fibulin 7 may have anti-angiogenic activity (3). Fibulin 7 may be important for proper development, since it is included in a chromosomal deletion that is associated with severe cardiac defects and craniofacial anomalies (4).
References:
Gallagher, W.M. et al. (2005) Trends Mol. Med. 11:336.
de Vega, S. et al. (2007) J. Biol. Chem. 282:30878.
de Vega, S. et al. (2014) Arch. Biochem. Biophys. 545:148.
Russell, M.W. et al. (2014) Hum. Mol. Genet. 23:4272.
Entrez Gene IDs:
129804 (Human); 70370 (Mouse); 296145 (Rat)
Alternate Names:
FBLN7; FIBL-7; Fibulin 7; TM14
