Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Mouse TGF- beta 2 (Catalog # ) is immobilized at 1 µg/mL (100 µL/well), the concentration of Recombinant Mouse Vasorin/SLIT-like 2 that produces 50% of the optimal binding response is approximately 0.3-1.8 μg/mL
Source
Mouse myeloma cell line, NS0-derived Cys25-Pro576, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
AnalysisCys25
Predicted Molecular Mass
61 kDa
SDS-PAGE
72-97 kDa, reducing conditions
8948-VN |
| |
Formulation Lyophilized from a 0.2 μm filtered solution in MOPS, NaCl, Ethylene Glycol and Trehalose. | ||
Reconstitution Reconstitute at 250 μg/mL in water. | ||
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | ||
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: Vasorin/SLIT-like 2
Vasorin, also known as SLIT-like 2, is an approximately 110 kDa transmembrane protein that is expressed on vascular smooth muscle cells and in the developing skeletal system (1, 2). It is down-regulated following vascular injury (1). A 95 kDa soluble form of Vasorin can be shed from the cell surface by ADAM17 mediated cleavage (3). The shed soluble form of Vasorin binds to TGF-beta 1, 2, and 3 and prevents TGF-beta induced effects including intimal formation and epithelial mesenchymal transition (1, 3). Soluble Vasorin is elevated in the serum of hepatic carcinoma patients relative to normal or non-cancerous hepatic disease (4). It promotes tumor cell proliferation, migration, and survival (4). Mature mouse Vasorin consists of a 552 amino acid (aa) extracellular domain (ECD) with 10 leucine rich repeats (LRR) flanked by one LRRNT and one LRRCT domain, one EGF-like domain, and one Fibronectin type-III domain, a 21 aa transmembrane segment, and a 76 aa cytoplasmic domain (1). Within the ECD, mouse Vasorin shares 85% and 96% aa sequence identity with human and rat Vasorin, respectively.
References:
Ikeda, Y. et al. (2004) Proc. Natl. Acad. Sci. USA 101:10732.
Krautzberger, A.M. et al. (2012) Gene Expr. Patterns 12:167.
Malapeira, J. et al. (2011) Oncogene 30:1912.
Li, S. et al. (2015) Oncotarget 6:10045.
Entrez Gene IDs:
114990 (Human); 246154 (Mouse)
Alternate Names:
Atia; Protein slit-like 2; SLIT like 2; Slitl2; slit-like 2 (Drosophila); slit-like 2; VASN; Vasorin
