Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to induce adhesion of ATDC5 mouse chondrogenic cells. The ED 50 for this effect is 0.15-0.9 μg/mL.
Source
Mouse myeloma cell line, NS0-derived Gln20-Val755, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
AnalysisNo results obtained. Gln20 inferred from enzymatic pyroglutamate treatment revealing Gly21
Structure / Form
Disulfide-linked pentamer
Predicted Molecular Mass
81 kDa
SDS-PAGE
85-115 kDa, reducing conditions
8958-CP |
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Formulation Lyophilized from a 0.2 μm filtered solution in Tris and NaCl with Trehalose | ||
Reconstitution Reconstitute at 500 μg/mL in PBS. | ||
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. | ||
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Data Images
Binding Activity
| Recombinant Mouse COMP/Thrombospondin-5 (Catalog # 8958-CP) induces adhesion of ATDC5 mouse chondrogenic cells. The ED50 for this effect is 0.15-0.9 μg/mL. |
Background: COMP/Thrombospondin-5
Cartilage Oligomeric Matrix Protein (COMP), also known as Thrombospondin-5, is a 110 kDa multidomain calcium binding protein that associates with other extracellular matrix molecules. Thrombospondin-1 and -2 constitute subgroup A and form homotrimers, whereas Thrombospondin-3, -4, and COMP constitute subgroup B and form homopentamers (1-4). Mouse COMP contains a non-collagenous coiled-coil domain, four EGF-like repeats, eight TSP type-3 repeats, and a globular TSP C-terminal domain (5). It shares 92% and 98% aa sequence identity with human and rat COMP, respectively. The coiled coil domain mediates the association of COMP into disulfide-linked homopentamers with a central hub and peripheral globular domains connected by flexible strands (6, 7). An axial pore is formed by the coiled coil assembly and binds vitamin D 3 which is involved in bone and cartilage metabolism (8). An RGD sequence in the third TSP type-3 repeat mediates chondrocyte attachment via Integrin alpha 5 beta 1, although when reduced and in the absence of calcium, attachment is mediated via Integrin alpha V beta 3 (9). COMP is up-regulated in rheumatoid arthritis and osteoarthritis, hepatocellular carcinomas, chronic pancreatitis, and pancreatic carcinomas (10-12). Elevated circulating COMP levels are used as a biomarker for early onset of some skeletal disorders (10). Several mutations are associated with skeletal dysplasias, and the most common, a point mutation in the third TSP type-3 repeat, results in diminished calcium binding ability (13, 14).
References:
Adams, J.C. and J. Lawler (2004) Int J. Biochem. Cell Biol. 36:961.
Posey, K.L. et al. (2014) Matrix Biol. 37:167.
Adams, J.C. (2004) Int. J. Biochem. Cell Biol. 36:1102.
Mann, H.H. et al. (2004) J. Biol. Chem. 279:25294.
Fang, C. et al. (2000) J. Orthop. Res. 18:593.
DiCesare, P. et al. (1995) J. Orthopaedic Res. 13:422.
Efimov, V.P. et al. (1994) FEBS Lett. 341:54.
Ozbek, S., et al. (2002) EMBO J. 21:5960.
Chen, F.H., et al. (2005) J. Biol. Chem. 280:32655.
Wislowska, M. and B. Jablonska (2005) Clin. Rheumatol. 24:278.
Xiao, Y. et al. (2004) J. Gastroenterol. Hepatol. 19:296.
Liao, Q. et al. (2003) Scand. J. Gastroenterol. 38:207.
Kennedy, J., et al. (2005) Eur. J. Hum. Genet. 13:547.
Hou, J. et al. (2000) Cell Calcium 27:309.
Long Name:
Cartilage Oligomeric Matrix Protein
Entrez Gene IDs:
1311 (Human); 12845 (Mouse)
Alternate Names:
cartilage oligomeric matrix protein (pseudoachondroplasia, epiphyseal dysplasia1, multiple); cartilage oligomeric matrix protein; cartilage oligomeric matrix protein(pseudoachondroplasia, epiphyseal dysplasia1, multiple); COMP; EDM1; EPD1; MED; MEDMGC131819; MGC149768; PSACH; pseudoachondroplasia (epiphyseal dysplasia 1, multiple); THBS5; Thrombospondin5; Thrombospondin-5; TSP5
