Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit Wnt-3a-induced alkaline phosphatase production by MC3T3‑E1 mouse preosteoblast cells. The ED 50 for this effect is 10-60 ng/ml in the presence of 5 ng/mL of Recombinant Mouse Wnt-3a (Catalog # ).
Source
Mouse myeloma cell line, NS0-derived
Mouse Frizzled-5
(Ala27-Pro167)
Accession # Q9EQD0IEGRMD Mouse IgG2a
(Glu98-Lys330)N-terminus C-terminus Accession #
N-terminal Sequence
AnalysisAla27
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
43 kDa
SDS-PAGE
54-64 kDa, reducing conditions
9060-FZ |
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Formulation Lyophilized from a 0.2 μm filtered solution in PBS. | ||
Reconstitution Reconstitute at 500 μg/mL in PBS. | ||
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | ||
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Data Images
Bioactivity
| Recombinant Mouse Frizzled-5 FC Chimera (Catalog # 9060-FZ) inhibits Recombinant Mouse Wnt-3a (Catalog # 1324-WN) induced alkaline phosphatase production in the MC3T3-E1 mouse preosteoblast cell line. The ED50 for this effect is 0.01-0.06 µg/ml. |
Background: Frizzled-5
Frizzled-5 is an approximately 62 kDa 7-TM glycoprotein that is one of at least ten Frizzled family Wnt receptors (1, 2). Wnt engagement of Frizzled, with low density lipoprotein receptor-related proteins LRP-5 or LRP-6 acting as co‑receptors, stabilizes beta -catenin and promotes gene transcription that is important in development and tissue maintenance (1). Frizzleds can also signal through non-canonical pathways independently of LRPs (1). Mature mouse Frizzled-5 consists of a 212 amino acid (aa) extracellular domain (ECD), a 283 aa 7-TM region, and a 64 aa cytoplasmic domain with a PDZ binding motif (2, 3). The ECD includes a cysteine-rich region (CRD) that binds Wnts and is highly conserved among Frizzled proteins (1, 2, 4). Within the N-terminal ECD, human Frizzled-5 shares 95% aa sequence identity with mouse and rat Frizzled-5. Frizzled-5 is expressed in embryonic tissues (telencephalon, pituitary, thalamus, hypothalamus, eye, liver, spleen, lung, and kidney), in the adult retina, colon, and pancreatic islets, some cancer cell lines, human embryonic stem cells, and in some monocyte and lymphocyte populations (2-8). Frizzled-5 functions as a receptor for Wnt-5a, Wnt-9b, Wnt-10b, Wnt-2b, and Wnt-7a (3, 5, 9, 10). It plays a role in the maintenance of yolk sac and placental vasculature (ishikawa) and in the regression of vitreous vasculature during eye development (5, 6, 11). It mediates the synaptogenic effect of Wnt-7a, contributes to the development of neuronal polarity, and is required for neuronal survival in the thalamus (3, 12, 13). In macrophages and monocytes, Frizzled-5 signaling induces the production of inflammatory cytokines (7, 8).
References:
Schulte, G. (2015) Eur. J. Pharmacol. 763:191.
Saitoh T. et al. (2001) Int. J. Oncol. 19:105.
Liu, C. et al. (2008) J. Neurosci. 28:5641.
Katoh, Y. and M. Katoh (2007) Int. J. Oncol. 30:751.
Ishikawa, T. et al. (2001) Development 128:25.
Burns, C.J. et al. (2008) Dev. Dyn. 237:1614.
Periera, C. et al. (2008) Arterioscler. Thromb. Vasc. Biol. 28:504.
Blumenthal, A. et al. (2006) Blood 108:965.
He, X. et al. (1997) Science 275:1652.
Caricasole, A. et al. (2003) J. Biol. Chem 278:37024.
Zhang, J. et al. (2008) Invest. Ophthalmol. Vis. Sci. 49:5561.
Sahores, M. et al. (2010) Development 137:2215.
Slater, P.G. et al. (2013) PLoS One 8:e78892.
Entrez Gene IDs:
7855 (Human); 14367 (Mouse)
Alternate Names:
C2orf31; chromosome 2 open reading frame 31; DKFZP434E2135; frizzled (Drosophila) homolog 5; frizzled homolog 5 (Drosophila); Frizzled5; Frizzled-5; Frz-5; FZ-5; FZD5; fzE5; HFZ5; MGC129692; seven-transmembrane receptor frizzled-5; Wnt receptor
