Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured in a cell proliferation assay using T11 mouse plasmacytoma cells. Nordan, R.P. et al. (1987) J. Immunol. 139:813. The ED 50 for this effect is 0.5-2 µg/mL in the presence of 0.75 ng/mL Recombinant Mouse IL‑11 (Catalog # )
Source
Mouse myeloma cell line, NS0-derived
Mouse IL-11 R alpha
(Met-Gln367)
Accession # NP_001156873DIEGRDP Mouse IgG2A
(Glu98-Lys330)N-terminus C-terminus Accession #
N-terminal Sequence
AnalysisCys26
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
64.6 kDa (monomer)
SDS-PAGE
75-80 kDa, reducing conditions
7405-MR |
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Formulation Lyophilized from a 0.2 μm filtered solution in PBS. | ||
Reconstitution Reconstitute at 100 μg/mL in PBS. | ||
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. | ||
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Background: IL-11 R alpha
Interleukin‑11 receptor alpha (IL‑11 R alpha, IL‑11 R alpha 1) originally designated NR1 in mouse, is a 49 kDa type I transmembrane protein that is a member of the gp130 subfamily of the hematopoietic cytokine receptor family (1‑4). Mouse IL‑11 R alpha cDNA encodes 432 amino acids (aa) including a 23 aa signal peptide, a 349 aa extracellular domain (ECD) that contains a C2 type Ig‑like domain, two fibronectin type III domains, two potential glycosylation sites and a WSXWS motif, a 21 aa transmembrane region and a short (39 aa) cytoplasmic domain (4). Mouse IL‑11 R alpha shares 84%, 95%, 85% and 82% aa sequence identity with human, rat, equine and bovine IL‑11 R alpha, respectively. In mouse, a second IL‑11 R (called alpha 2 or beta ) that differs by only 4 aa is expressed primarily in the testes (5). IL-11 R alpha first binds IL‑11 with low affinity, then forms a high affinity receptor when complexed with gp130 homodimers (1, 3). IL‑11 R alpha is widely expressed in adults, embryos and embryonic stem cells (4‑6). Deletion in female mice causes faulty decidualization and lack of decidual NK cells and results in infertility (7‑9). IL-11 is anti-apoptotic for oligodendrocytes, and lack of IL‑11 R alpha increases the severity of experimental autoimmune encephalitis (10, 11). IL‑11 R alpha is also anti-apoptotic for colonic epithelia, and increased IL‑11 signaling may be a factor in inflammation-associated gastrointestinal cancer development (3, 12). IL‑11 R alpha enhances osteoclast differentiation and bone remodeling, but inhibits adipocyte differentiation (1, 2). Recombinant soluble IL‑11 R alpha confers IL‑11 responsiveness to cells expressing gp130, while in cells expressing transmembrane IL‑11 R alpha and gp130, soluble IL‑11 R alpha acts as an IL‑11 antagonist (13‑15).
References:
Sims, N.A. and N.C. Walsh (2010) BMB Reports 43:513.
White, U.A. and J.M. Stephens (2011) Curr. Pharm. Des. 17:340.
Kiessling, S. et al. (2004) J. Biol. Chem. 279:10304.
Hilton, D.J. et al. (1994) EMBO J. 13:4765.
Bilinski, P. et al. (1996) Biochem. J. 320:359.
Davidson, A.J. et al. (1997) Stem Cells 15:119.
Paiva, P. et al. (2009) Cytokine Growth Factor Rev. 20:319.
Robb, L. et al. (1998) Nat. Med. 4:303.
Ain, R. et al. (2004) Dev. Dyn. 231:700.
Gurfein, B.T. et al. (2009) J. Immunol. 183:4229.
Zhang, J. et al. (2011) J. Immunol. 187:1129.
Putoczki, T. and M. Ernst (2010) J. Leukoc. Biol. 88:1109.
Curtis, D.J. et al. (1997) Blood 90:4403.
Baumann, H. et al. (1996) J. Immunol. 157:284.
Karow, J. et al. (1996) Biochem. J. 318:489.
Long Name:
Interleukin 11 Receptor alpha
Entrez Gene IDs:
3590 (Human); 16157 (Mouse)
Alternate Names:
AI314697; GP130; IL-11 R alpha; IL11R alpha; IL11RA; IL-11Ra; Il11ra2; NR1
