Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to induce IL-6 secretion by NIH‑3T3 mouse embryonic fibroblast cells. Towne, J.E. et al. (2004) J. Biol. Chem. 279:13677. The ED 50 for this effect is typically 3-18 ng/mL.
Source
E. coli-derived Arg8-His160
Accession #
N-terminal Sequence
AnalysisArg8
Predicted Molecular Mass
17 kDa
SDS-PAGE
16.5 kDa, reducing conditions
Carrier Free
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
7059-ML |
| 7059-ML/CF |
Formulation Lyophilized from a 0.2 μm filtered solution in MOPS, NaCl, TCEP and EDTA with BSA as a carrier protein. | Formulation Lyophilized from a 0.2 μm filtered solution in MOPS, NaCl, TCEP and EDTA with Trehalose. | |
Reconstitution Reconstitute at 100 μg/mL in PBS containing at least 0.1% human or bovine serum albumin. | Reconstitution Reconstitute at 100 μg/mL in PBS. | |
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. | Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | |
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
| Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Data Images
Bioactivity
| Recombinant Mouse IL-36 alpha /IL-1F6 (aa 8‑160) (Catalog # 7059‑ML) induces IL‑6 secretion in the NIH‑3T3 mouse embryonic fibroblast cell line. The ED50 for this effect is 3-18 ng/mL. |
SDS-PAGE
| 1 μg/lane of Recombinant Mouse IL‑36 alpha /IL‑1F6 (aa 8-160) was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 17 kDa. |
Background: IL-36 alpha/IL-1F6
IL‑36 alpha, previously called IL‑1F6 and FIL1 epsilon (family of IL‑1 member epsilon), is a member of the IL‑1 family which includes IL‑1 beta, IL‑1 alpha, IL‑1ra, IL‑18, and novel family members IL‑36 Ra (IL‑1F5), IL‑36 beta (IL‑1F8), IL‑36 gamma (IL‑1F9), IL‑37 (IL‑1F7) and IL‑1F10 (14). All family members show a 12 beta ‑strand, beta ‑trefoil configuration, and are believed to have arisen from a common ancestral gene (1, 2). IL‑36 alpha is an 18‑22 kDa, 160 amino acid (aa) intracellular and secreted protein that contains no signal sequence, no prosegment and no potential N‑linked glycosylation sites (1‑3). It can be externalized non‑specifically in response to LPS and ATP‑induced activation of the P2X7 receptor (5). Full‑length recombinant IL‑36 alpha is less active than endogenous IL‑36 alpha, but trimming of the N‑termini enhances its activity (6). Mouse IL‑36 alpha (aa 8‑160) shares 83% aa sequence identity with rat IL‑36 alpha, 54‑60% with human, rabbit, equine and bovine IL‑36 alpha, and 27‑57% aa sequence identity with other novel IL‑1 family members. IL‑36 alpha is mainly found in skin and lymphoid tissues, but also in fetal brain, trachea, stomach and intestine (1, 3, 7). It is expressed by monocytes, B and T cells (1, 2). The receptor for IL‑36 alpha is a combination of IL‑1 Rrp2 (also called IL‑1 RL2 or IL‑1 R6), mainly found in epithelia and keratinocytes, and the widely expressed IL‑1 RAcP (3, 6, 7). IL‑36 alpha, beta and gamma all activate NF‑ kappa B and MAPK pathways in an IL‑1 Rrp2 dependent manner, and induce production of inflammatory cytokines and chemokines such as CXCL8/IL‑8 (7). IL‑36 alpha and other family members are overexpressed in psoriatic skin lesions, and transgenic overexpression of IL‑36 alpha in skin keratinocytes produces epidermal hyperplasia (6‑9). IL‑36 alpha is present in kidney tubule epithelia; it is highly overexpressed in tubulointerstitial lesions in mouse models of chronic glomerulonephritis, lupus nephritis and diabetic nephritis (10). IL‑36 alpha is induced by inflammation in adipose tissue‑resident alternately activated (M2) macrophages, and reduces adipocyte differentiation (11).
References:
Smith, D.E. et al. (2000) J. Biol. Chem. 275:1169.
Dunn, E. et al. (2001) Trends Immunol. 22:533.
Barksby, H.E. et al. (2007) Clin. Exp. Immunol. 149:217.
Dinarello, C. et al. (2010) Nat. Immunol. 11:973.
Martin, U. et al. (2009) J. Immunol. 183:4021.
Blumberg, H. et al. (2007) J. Exp. Med. 204:2603.
Towne, J.E. et al. (2004) J. Biol. Chem. 279:13677.
Blumberg, H. et al. (2010) J. Immunol. 185:4354.
Johnston, A. et al. (2011) J. Immunol. 186:2613.
Ichii, O. et al. (2010) Lab. Invest. 90:459.
van Asseldonk, E.J.P. et al. (2010) Obesity 18:2234.
Long Name:
Interleukin 36 alpha/Interleukin 1 Family 6
Entrez Gene IDs:
27179 (Human); 54448 (Mouse)
Alternate Names:
FIL1 epsilon; FIL1; FIL1(EPSILON); FIL1E; IL-1 epsilon; IL1(EPSILON); IL1E; IL-1E; IL-1F6 (FIL-1-epsilon); IL1F6; IL-1F6; IL36 alpha; IL-36 alpha; IL36A; interleukin 1 family, member 6 (epsilon); interleukin 1, epsilon; Interleukin 36, Alpha; Interleukin-1 epsilon; interleukin-1 family member 6; Interleukin-36 Alpha
