Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to induce adhesion of ATDC5 mouse chondrogenic cells. The ED 50 for this effect is 0.6-3.6 µg/mL.
Source
Human embryonic kidney cell, HEK293-derived Asn24-Ser800, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
AnalysisAsn24
Predicted Molecular Mass
87 kDa
SDS-PAGE
77-95 kDa, reducing conditions
8994-F2 |
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Formulation Lyophilized from a 0.2 μm filtered solution in MES, NaCl and Brij-35. | ||
Reconstitution Reconstitute at 500 μg/mL in PBS. | ||
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | ||
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Background: Periostin/OSF-2
Periostin, also known as OSF-2, is a secreted matricellular protein with functions in extracellular matrix formation, cell migration, and inflammation (1). It is secreted as a 90 kDa monomer that can aggregate into >170 kDa higher-order multimers (2). Periostin contains an N-terminal EMI domain followed by four tandem FAS1 domains (3). Alternative splicing generates additional isoforms of rat Periostin with various deletions in the C-terminal region following the FAS domains. This recombinant protein corresponds to the isoform of rat Periostin that lacks aa 785-812. It shares 90% and 97% aa sequence identity with comparable isoforms of human and mouse Periostin, respectively. Periostin is expressed by mesenchymal cells such as vascular smooth muscle cells, fibroblasts, osteoblasts, and odontoblasts in developing teeth (4-7). It is up-regulated in many carcinomas (2, 8). Periostin binds to Integrins alpha v beta 3 and alpha v beta 5 (2, 9), leading to enhanced cell adhesion and cell migration (2, 5, 6). It enhances Fibronectin and Collagen I production and promotes collagen fibrillogenesis (10, 11). It also induces epithelial-mesenchymal transition, tumor growth, invasion, and metastasis (9). Periostin induces the expression of VEGF R2 on endothelial cells and VEGF-C in tumor cells, and it can induce tumor lymphangiogenesis (8, 12). Periostin plays an important role in heart valve development and tissue healing after myocardial infarction (5, 13, 14). In asthma, it is
up-regulated in bronchial epithelium and plays both destructive and protective roles by inducing eosinophil infiltration and inhibiting goblet cell metaplasia and mucus production, respectively (15, 16).
References:
Liu, A.Y. et al. (2014) Matrix Biol. 37:150.
Gillan, L. et al. (2002) Cancer Res. 62:5358.
Takeshita, S. et al. (1993) Biochem. J. 294:271.
Kruzynska-Frejtag, A. et al. (2004) Dev. Dyn. 229:857.
Lindner, V. et al. (2005) Arterioscler. Thromb. Vasc. Biol. 25:77.
Horiuchi, K. et al. (1999) J. Bone Miner. Res. 14:1239.
Li, G. et al. (2006) Atherosclerosis 188:292.
Shao, R. et al. (2004) Mol. Cell. Biol. 24:3992.
Yan, W. and R. Shao (2006) J. Biol. Chem. 281:19700.
Erkan, M. et al. (2007) Gastroenterology 132:1447.
Norris, R.A. et al. (2007) J. Cell. Biochem. 101:695.
Kudo, Y. et al. (2012) PLoS One 7:e44488.
Snider, P. et al. (2008) Circ. Res. 102:752.
Kuhn, B. et al. (2007) Nat. Med. 13:962.
Blanchard, C. et al. (2008) Mucosal Immunol. 1:289.
Sehra, S. et al. (2011) J. Immunol. 186:4959.
Long Name:
Osteoblast Specific Factor 2
Entrez Gene IDs:
10631 (Human); 50706 (Mouse); 361945 (Rat)
Alternate Names:
Fasciclin I-like; MGC119510; MGC119511; OSF2; OSF-2; OSF-2osteoblast specific factor 2 (fasciclin I-like); OSF2periodontal ligament-specific periostin; Osteoblast-specific factor 2; PDLPOSTN; periostin isoform thy2; periostin isoform thy4; periostin isoform thy6; periostin isoform thy8; Periostin; periostin, osteoblast specific factor; PNRP11-412K4.1; POSTN; TRIF52
