Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit neurite outgrowth of dissociated E13 chick embryonic dorsal root ganglia (DRG) neurons. Able to significantly inhibit neurite outgrowth when immobilized at 3 μg/mL on a nitrocellulose-coated microplate.
Source
Mouse myeloma cell line, NS0-derived
Rat Nogo-A
(Met1-Val172)
Accession # Q9JK11IEGRMDP Mouse IgG2A
(Glu98-Lys330)N-terminus C-terminus Accession #
N-terminal Sequence
AnalysisMet1
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
45 kDa (monomer)
SDS-PAGE
66-76 kDa, reducing conditions
3098-NG |
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Formulation Lyophilized from a 0.2 μm filtered solution in PBS. | ||
Reconstitution Reconstitute at 400 μg/mL in PBS. | ||
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | ||
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Background: Nogo-A
Nogo, so named as a “No-Go” for neurite outgrowth, is a member of the reticulon family of transmembrane proteins, and is also called reticulon 4 (gene name RTN4) (1-4). Reticulons lack N-terminal signal sequences, share a conserved ~200 amino acid (aa) C-terminus that contains two transmembrane domains and an ER‑retention motif, and show a punctate intracellular distribution within the endoplasmic reticulum (ER) that is reminescent of a reticulum (1-3). The N-terminus of intracellular (ER) Nogo-A appears to face the cytoplasm (1-3). However, minor amounts of Nogo-A and Nogo-B are found in the plasma membrane with extracellular N-termini (4-6). Full length rat Nogo-A is a 1163 aa protein with a long (~989 aa) N-terminus that includes bioactive regions (aa 59-172 and 544-725), a transmembrane segment, a connecting loop that contains the bioactive Nogo-66 region, a second transmembrane segment, and a short C-terminus (3). The four Nogo isoforms share the Nogo66 segment, Nogo-A and Nogo-B share aa 1-172, and only Nogo-A contains aa 544-725 (1-3). Rat Nogo-A shares 78% and 91% aa sequence identity with human and mouse Nogo-A, respectively. Rat and human Nogo-A/B also share 78% aa sequence identity within aa 1-172 and 98% within the Nogo-66 loop region. Nogo-A is mainly expressed in oligodendrocytes of the central nervous system, but is also reported in fibroblasts, dorsal root ganglion neurons, macrophages and myoblasts (1-8). Nogo-B is mainly expressed in vascular endothelium and smooth muscle throughout the body (1, 4, 6, 9). The Nogo66 region binds the GPI-linked Nogo receptor/p75 complex on axons, inducing growth cone collapse (5, 7, 10, 11). Either aa 59-172 or 544-725 segments can block neurite outgrowth and fibroblast spreading (5, 6). Nogo-A/B aa 1-172 is also reported to regulate vascular remodeling through binding the Nogo-B receptor (NgBR/NUS1) on vascular cells, and to inhibit neuronal differentiation and promote glial formation from neural progenitors (4, 5, 8, 9, 12).
References:
Oertle, T. and M.E. Schwab (2003) Trends Cell Biol. 13:187.
GrandPre, T. et al. (2000) Nature 403:439.
Chen, M.S. et al. (2000) Nature 403:434.
Acevedo, L. et al. (2004) Nat. Med. 10:382.
Oertle, T. et al. (2003) J. Neurosci. 23:5393.
Dodd, D.A. et al. (2005) J. Biol. Chem. 280:12494.
Wang, X. et al. (2002) J. Neurosci. 22:5505.
Schwab, M.E. (2010) Nat. Rev. Neurosci. 11:799.
Miao, R.Q. et al. (2006) Proc. Natl. Acad. Sci. USA 103:10997.
Fournier, A.E. et al. (2001) Nature 409:341.
Wang, K.C. et al. (2002) Nature 420:74.
Guo, Y. et al. (2009) Neurosci. Lett. 458:132.
Long Name:
Reticulon 4A
Entrez Gene IDs:
57142 (Human); 68585 (Mouse); 83765 (Rat)
Alternate Names:
NI220; NogoA; Nogo-A; RTN4; RTN4A
