Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to induce alkaline phosphatase production by ATDC5 mouse chondrogenic cells. Nakamura, K. et al. (1999) Exp. Cell Res. 250:351. The ED 50 for this effect is 0.2-1 µg/mL.
Source
E. coli-derived Ser288-Arg400, with an N-terminal Met
Accession #
N-terminal Sequence
AnalysisMet
Predicted Molecular Mass
12.9 kDa
Carrier Free
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
1128-BM |
| 1128-BM/CF |
Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein. | Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA. | |
Reconstitution Reconstitute at 100 μg/mL in sterile 4 mM HCl containing at least 0.1% human or bovine serum albumin. | Reconstitution Reconstitute at 100 μg/mL in sterile 4 mM HCl. | |
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | |
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
| Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: BMP-4
BMP-4 is one of at least 15 structurally and functionally-related BMPs, which are members of the transforming growth factor-beta (TGF-beta ) superfamily. BMPs were originally identified as protein regulators of cartilage and bone formation. They have since been shown to have roles in embryogenesis and morphogenesis of various tissues and organs. BMPs have also been shown to regulate the growth, differentiation, chemotaxis, and apoptosis of various cell types, including mesenchymal cells, epithelial cells, hematopoietic cells, and neuronal cells. Similarly to other TGF-beta superfamily proteins, BMPs are highly conserved across animal species. Zebrafish BMP-4 protein shares 73% amino acid sequence identity with human and murine BMP-4, and 86% amino acid sequence identity with zebrafish BMP-2b. Zebrafish BMP-4 mRNA is detected throughout embryonic development and has been detected in tissues of adult fish, including the brain, heart, digestive tracts, testes, and jaw. BMP-4 has been shown to play roles in directing zebrafish heart looping and in ear development. The combined expression pattern of zBMP-2a/2b/4 coincides with areas where BMP-2/4 expression would be found in other vertebrates. Biologically active BMP-4 is a disulfide-linked homodimer of the carboxy-terminal 113 amino acid residues that contains the characteristic seven conserved cysteine residues involved in the formation of the cysteine knot and the single interchain disulfide bond.
References:
Kawabata, M. et al. (1998) Cytokine and Growth Factor Reviews 9:49.
Hwang, S.P. et al. (1997) DNA Cell Biol. 16:1003.
Martinez-Barbera, J.P. et al. (1997) Gene 197:53.
Mowbray, C. et al. (2001) Mech Dev. 108:179.
Chen J.N. et al. (1997) Development 124:4373.
Long Name:
Bone Morphogenetic Protein 4
Entrez Gene IDs:
652 (Human); 12159 (Mouse); 25296 (Rat); 30612 (Zebrafish)
Alternate Names:
BMP-2B; BMP2B1; BMP2BMCOPS6; BMP4; BMP-4; Bone morphogenetic protein 2B; bone morphogenetic protein 4; DVR4; OFC11; ZYME
