Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with mouse CXCR3. The ED 50 for this effect is 2-10 µg/mL.
Source
E. coli-derived Ile22-Val94, with an N-terminal Met
Accession #
N-terminal Sequence
AnalysisMet
Predicted Molecular Mass
8.6 kDa
1117-IP |
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Formulation Lyophilized from a 0.2 μm filtered solution in PBS. | ||
Reconstitution Reconstitute at 100 μg/mL in sterile PBS. | ||
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | ||
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Background: CXCL10/IP-10/CRG-2
P-10, also named mob-1 in rat and crg-2 in mouse, is a member of the non-ELR-motif containing CXC chemokine subfamily and has been designated CXCL10. It is induced in various cell types (including keratinocytes, endothelial cells, fibroblasts, smooth muscle cells, monocytes/macrophages, astrocytes, neutrophils, hepatocytes, T cells, epithelial cells and mesothelial cells) in response to stimulation by type I and/or type II interferon as well as by LPS. IP-10 has been shown to chemoattract activated CD4+ and CD8+ T cells, activated NK cells and smooth muscle cells. It also induces the adhesion of activated T cells to VCAM and ICAM. In addition, IP-10 has been reported to antagonize the angiogenic activity of FGF. IP-10 binds to and activates CXCR3, the chemokine receptor that is shared by MIG/CXCL9 and I-TAC/CXCL11, two additional non-ELR CXC chemokines that are induced by interferon. Cotton rat IP-10 cDNA encodes a 94 amino acid (aa) residue precursor protein with a putative 21 aa signal peptide and a 73 aa mature protein with one potential N-linked glycosylation site. Mature cotton rat IP-10 shares 66%, 64%, and 66% aa sequence identity with rat, mouse and human IP10, respectively (1 - 7).
References:
Vanguri, P. and J.M. Farber (1990) J. Biol. Chem. 265:15049.
Liang, P. et al. (1994) Proc. Natl. Acad. Sci. USA. 91:12515.
Proost, P. et al. (1993) J. Immunol. 150:1000.
Blanco, J.C. et al. (2001) GenBank Accession # AAL16959.
Neville, L.F. (1997) Prog. Growth Factor Res. 8:207.
Belperio, J.A. et al. (2000) J. Leukoc. Biol. 68:1.
Soejima, K. and B.J. Rollins (2001) J. Immunol. 167:6576.
Entrez Gene IDs:
3627 (Human); 15945 (Mouse)
Alternate Names:
C7; chemokine (C-X-C motif) ligand 10; CRG2; CRG-2; CXCL10; gIP-10; IFI10; INP10; IP-10; mob-1; SCYB10
