Purity
>95%, by SDS-PAGE with silver staining
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Human R‑Spondin 3 (Catalog # ) is coated at 0.25 μg/mL, Recombinant Human ZNRF3 Fc Chimera binds with an apparent K D <3 nM.
Source
Chinese Hamster Ovary cell line, CHO-derived
Human ZNRF3
(Lys56-Met219)
Accession # Q9ULT6IEGRMD Human IgG1
(Pro100-Lys330)N-terminus C-terminus Accession #
N-terminal Sequence
AnalysisLys56
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
44.8 kDa (monomer)
SDS-PAGE
50-55 kDa, reducing conditions
7994-RF |
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Formulation Lyophilized from a 0.2 μm filtered solution in PBS. | ||
Reconstitution Reconstitute at 500 μg/mL in PBS. | ||
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | ||
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Background: ZNRF3
ZNRF3 (zinc/RING finger protein 3), also called RNF203, is a 101 kDa (predicted) type I transmembrane protein that is a member of the ZNRF3 family of ubiquitin ligases (1). Human ZNRF3 is synthesized as a 936 amino acid (aa) protein that contains a putative 55 aa signal sequence, a 164 aa extracellular domain (ECD), a transmembrane domain, and a cytoplasmic domain with an atypical RING (really interesting new gene) -type zinc finger (1). The mature human ZNRF3 ECD shares 98% aa sequence identity with mouse ZNRF3. An alternative start site at Met101 creates a 90 kDa (predicted) form; the shorter ECD of this human isoform shares 100% aa sequence identity with canine, feline and equine, 99% with porcine, and 98% with rat ZNRF3 (1). In the plasma membrane, ZNRF3 is co‑expressed with the related protein RNF43, and both serve as E3 ubiquitin ligases that promote turnover of Frizzled Wnt receptors and LRP6 to antagonize Wnts (2‑4). Interaction with the R‑Spondin Wnt‑regulating proteins antagonizes ZNRF3, thus stabilizing Frizzleds and LRP6, allowing their accumulation, and promoting Wnt signaling (2, 3). ZNRF3 is expressed in stem cells at the bottom of colon crypts, where it limits the ability of Wnts to induce proliferation (4). It is also expressed in normal gastric tissues, but is down‑regulated in gastric adenocarcinomas (5).
References:
SwissProt Accession # Q9ULT6.
MacDonald, B.T. and X. He (2012) Cell Res. 22:1410.
Hao, H.X. et al. (2012) Nature 485:195.
Koo, B.K. et al. (2012) Nature 488:665.
Zhou, Y. et al. (2013) J. Mol. Histol. Mar 17 [Epub ahead of print].
Long Name:
Zinc and Ring Finger 3
Entrez Gene IDs:
84133 (Human); 407821 (Mouse)
Alternate Names:
BK747E2.3; KIAA1133; novel C3HC4 type Zinc finger (ring finger); RNF203; zinc and ring finger 3; zinc/RING finger protein 3; ZNRF3
