Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to induce p38 MAPK activation in 3T3‑L1 mouse embryonic fibroblast adipose-like cells. 1 μg/mL of Recombinant Human Irisin can effectively induce p38 MAPK activation.
Source
Chinese Hamster Ovary cell line, CHO-derived Asp32-Glu143, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
AnalysisAsp32
Predicted Molecular Mass
13 kDa
SDS-PAGE
15-37 kDa, reducing conditions
Carrier Free
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
8880-IR/CF |
| 8880-IR |
Formulation Lyophilized from a 0.2 μm filtered solution in MOPS, NaCl and TCEP. | Formulation Lyophilized from a 0.2 μm filtered solution in MOPS, NaCl and TCEP with BSA as a carrier protein. | |
Reconstitution Reconstitute at 100 μg/mL in PBS. | Reconstitution Reconstitute at 100 μg/mL in PBS containing at least 0.1% human or bovine serum albumin. | |
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | |
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
| Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Data Images
Recombinant Human Irisin Induces p38 MAPK Phosphorylation in 3T3-L1 Preadipocytes.
| 3T3-L1 preadipocytes were incubated with 1 µg/ml of RecombinantHuman Irisin for 15 minutes. The cellswere lysed and Western blots were performed with anti-phospho p38 MAPK andtotal p38 MAPK antibodies. Experiments are shown in duplicate. |
Background: Irisin/FNDC5
Irisin (also known as FNDC5) is a 12 kDa glycosylated polypeptide hormone that regulates energy metabolism, stem cell differentiation, and neuronal development (1, 2). Human Irisin is synthesized as a 212 amino acid (aa) precursor encoding a type 1 transmembrane protein with a 121 aa extracellular domain (ECD), a 21 aa transmembrane domain, and a 39 aa cytoplasmic domain. The ECD of Irisin contains a fibronectin type III domain and multiple glycosylation sites. The ECD is proteolytically cleaved to release the 112 aa soluble Irisin hormone into circulation (2-5). Mature human, mouse, and rat Irisin share 100% sequence identity. Expression of Irisin is induced in skeletal muscle and subcutaneous adipose tissue during and shortly after exercise (2, 6). Irisin induces expression of peroxisome proliferator-activated receptor gamma co-activator 1 alpha (PGC1 alpha ) and uncoupling protein-1 (UCP1), mitochondrial-associated metabolic proteins (7, 8). Irisin induces the transition of white adipose tissue into more metabolically active beige adipose tissue. In mice, expression of Irisin has been shown to regulate obesity and diabetes (1, 2). A similar function in humans is suggested (9). Irisin also regulates neuronal cell differentiation and neurite outgrowth in the brain and is involved in the differentiation of osteoblasts (10-14).
References:
Long Name:
Fibronectin Type III Domain Containing 5
Entrez Gene IDs:
252995 (Human); 384061 (Mouse); 260327 (Rat)
Alternate Names:
fibronectin type III domain containing 5; fibronectin type III domain-containing protein 5; FNDC5; FRCP2; FRCP2Fibronectin type III repeat-containing protein 2; Irisin
