Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to promote the expansion of E16 rat liver mononuclear cells in vitro, in the presence of Recombinant Mouse SCF/c‑kit Ligand (Catalog # ), Recombinant Mouse Thrombopoietin/Tpo (Catalog # ), and Recombinant Mouse Flt‑3 Ligand (Catalog # ). The ED 50 for this effect is 50-200 ng/mL in the presence of a cross‑linking antibody, Mouse Anti-polyHistidine Monoclonal Antibody (Catalog # ).
Source
Chinese Hamster Ovary cell line, CHO-derived Met1-Pro346, with a C-terminal 10-His tag
Accession #
N-terminal Sequence
AnalysisNo results obtained: Gln27 predicted
Predicted Molecular Mass
38.6 kDa
SDS-PAGE
49-55 kDa, reducing conditions
Carrier Free
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
914-AN |
| 914-AN/CF |
Formulation Lyophilized from a 0.2 μm filtered solution in PBS and NaCl with BSA as a carrier protein. | Formulation Lyophilized from a 0.2 μm filtered solution in PBS and NaCl. | |
Reconstitution Reconstitute at 100 μg/mL in PBS. | Reconstitution Reconstitute at 100 μg/mL in PBS. | |
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | |
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
| Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: Angiopoietin-like Protein 7/ANGPTL7
Angiopoietin-like 7 (ANGPTL7), also known as Corneal-Derived Transcript 6 (CDT6), is a secreted glycoprotein that is structurally related to the angiopoietins. Members of this protein family contain an N-terminal coiled coil domain and a C-terminal fibrinogen-like domain (1 - 3). ANGPTL7 shares 89% aa sequence identity with mouse and rat ANGPTL7. It is secreted as a 45 ‑ 50 kDa monomer that forms disulfide-linked homotrimers and tetramers via the coiled coil domain (4, 5). ANGPTL7 is expressed in the corneal stroma, trabecular meshwork, and sclera and is elevated in glaucoma aqueous humor (3, 5). Its production is up‑regulated in trabecular meshwork cells by glucocorticoids and TGF-beta and in cartilage by TNF-alpha (5 - 7). Overexpression of ANGPTL7 in trabecular meshwork cells inhibits the production of collagen and proteoglycans (8). When overexpressed in tumor cells it promotes collagen and proteoglycan deposition but inhibits tumor xenograft progression and tumor angiogenesis (4). Recently, ANGPTL proteins, including ANGPTL-2, -3, -4, -5 and -7, are identified as growth factors in combination with SCF, Thrombopoietin, IGF-II and FGF acidic that enhance the expansion and engraftment of human and mouse hematopoietic stem cells (9).
References:
Oike, Y. et al. (2009) Circ. J. 73:2192.
Miida, T. and S. Hirayama (2010) Curr. Opin. Lipidol. 21:70.
Peek, R. et al. (1998) Invest. Opthalmol. Visual Sci. 39:1782.
Peek, R. et al. (2002) J. Biol. Chem. 277:686.
Kuchtey, J. et al. (2008) Invest. Opthalmol. Visual Sci. 49:3438.
Nehme, A. et al. (2009) BMC Med. Genomics 2:58.
Stevens, A.L. et al. (2008) Arthritis Rheum. 58:489.
Comes, N. et al. (2010) Genes Cells 16:243.
Zhang, C.C. et al. (2006) Nat. Med. 12:240.
Entrez Gene IDs:
10218 (Human); 654812 (Mouse)
Alternate Names:
angiopoietin-like 7; Angiopoietin-like factor; Angiopoietin-like Protein 7; angiopoietin-related protein 7; ANGPTL7; AngX; CDT6; CDT6angiopoietin-like factor (CDT6); Cornea-derived transcript 6 protein; dJ647M16.1; RP4-647M16.2
