Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. Immobilized rhIL-20 Ra/Fc Chimera at 1 µg/mL (100 µL/well) can bind rhIL-26 with a linear range of 0.3-20 ng/mL.
Source
Mouse myeloma cell line, NS0-derived
Human IL-20 R alpha
(Val30 - Lys250)
Accession # Q9UHF4DIEGRMD Human IgG1
(Pro100 - Lys330)N-terminus C-terminus Accession #
N-terminal Sequence
AnalysisVal30
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
52 kDa (monomer)
SDS-PAGE
70-85 kDa, reducing conditions
1176-IR |
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Formulation Lyophilized from a 0.2 μm filtered solution in PBS. | ||
Reconstitution Reconstitute at 100 μg/mL in sterile PBS. | ||
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | ||
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Background: IL-20 R alpha
IL-20 receptor alpha (IL-20 R alpha ), also named IL-20 R1, CRF2-8, and ZCYTOR7, belongs to the class II cytokine receptor family, which includes 12 members. These receptors are characterized by the patterns of conserved amino acid (aa) residues in their extracellular domains, which are composed of tandem fibronectin type III domains (1). Class II cytokine receptors form heterodimeric signaling receptor complexes that mediate class II cytokine signals. Subunits of the different receptor complexes are shared and serve multiple functions (1).
The gene for human IL-20 R alpha is mapped to chromosome 6 and encodes a 553 aa glycoprotein with a 29 aa signal peptide, a 221 aa extracellular domain, a 24 aa transmembrane region and a 279 aa intracellular domain (2). IL-20 R alpha is widely expressed and is detected at high levels in multiple tissues including skin, testis, heart, placenta, salivary gland and prostate gland (1). The expression of IL-20 R alpha, together with that of IL-20 R beta, is upregulated in psoriatic skin lesions on keratinocytes, immune cells, and endothelial cells (1, 2).
IL-20 R alpha heterodimerizes with IL-20 R beta to form the functional receptor that mediates IL-19, IL-20 and IL-24 signals (3, 4). IL-20 R alpha also heterodimerizes with IL-10 R beta to form the functional receptor complex for IL-26 (5). Binding of these IL-10 family class II cytokines to their functional receptors induces activation of the JAK-STAT signal transduction pathway. At low ligand concentrations, STAT3 has been shown to be the predominant STAT proteins activated through either complexes (3 - 5).
References:
Kotenko, S.V. (2003) Cytokine & Growth Factor Reviews 13:223.
Xie, M.H. et al. (2000) J. Biol. Chem. 275:31335.
Dumoutier, L. et al. (2001) J. Immunol. 167:3534.
Parrish-Novak, J. et al. (2002) J. Biol. Chem. 277:47517s.
Sheikh. F. et al. (2004) J. Immunol. 172:2006.
Long Name:
Interleukin 20 Receptor alpha
Entrez Gene IDs:
53832 (Human); 237313 (Mouse)
Alternate Names:
class II cytokine receptor ZCYTOR7; CRF2-8; Cytokine receptor class-II member 8; Cytokine receptor family 2 member 8; FLJ40993; IL-20 R alpha; IL20R alpha; IL-20R1IL-20 receptor subunit alpha; IL20RA; IL-20Ra; IL-20R-alpha; interleukin 20 receptor, alpha; interleukin-20 receptor I; interleukin-20 receptor subunit alpha; zcytor7
