Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Activity
Measured by the ability of the immobilized protein to enhance BMP-2 induced alkaline phosphatase activity in C3H10T1/2 mouse embryonic fibroblast cells. Nobta, M. et al. (2005) J. Biol. Chem. 280:15842. The ED 50 for this effect is 0.5-2.0 μg/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived
Human Jagged 1
(Ser32-Ser1046)
Accession # P78504IEGRMD Human IgG1
(Pro100-Lys330)N-terminus C-terminus Accession #
N-terminal Sequence
AnalysisSer32
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
137 kDa
SDS-PAGE
170-180 kDa, under reducing conditions
1277-JG |
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Formulation Lyophilized from a 0.2 μm filtered solution in PBS and EDTA. | ||
Reconstitution Reconstitute at 200 μg/mL in sterile PBS. | ||
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | ||
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Background: Jagged 1
Jagged 1 is a 180 kDa type I transmembrane glycoprotein and member of the Delta-Serrate-Lag-2 (DSL) family of ligands that activate LIN12/Notch proteins. Human Jagged 1 is synthesized as a 1218 amino acid (aa) precursor that contains a 33 aa signal sequence, a 1034 aa extracellular domain (ECD), a 26 aa transmembrane segment, and a 125 aa cytoplasmic region (SwissProt # P78504). The ECD contains a DSL domain (aa 185-229), a cysteine-rich region, 15 EGF-like repeats, of which many bind calcium, and nine potential sites for N-linked glycosylation. Mature human Jagged 1 is 97% and 96% aa identical to mature mouse and rat Jagged 1, respectively. Jagged 1 is widely expressed in adult and fetal tissues. Jagged-Notch signaling specifies cell fate, regulates pattern formation, defines boundaries between different cell types, and modulates cell proliferation and differentiation, especially during hematopoiesis, myogenesis, neurogenesis, and development of vasculature (1-8). Mutations in human Jagged 1 are the cause of Alagille syndrome, an autosomal-dominant disorder characterized by intrahepatic cholestasis and abnormalities of heart, eye, vertebrae, as well as characteristic facial appearance (9-10).
References:
Sainson, R.C. and A.L. Harris (2008) Angiogenesis 11:41.
Cordle, J. et al. (2008) Nat. Struct. Mol. Biol. 15:849.
Artavamis-Tsokanas, S. et al. (1999) Science 284:770.
Lai, E.C. (2004) Development 131:965.
Milner, L.A. et al. (1994) Blood 83:2057.
Nyfeler, Y. et al. (2005) EMBO J. 24:3504
Linheng, L. et al. (1998) Immunity 8:43.
Iso, T. et al. (2003) Arterioscler. Thromb. Vasc. Biol. 23:543.
Oda, T. et al. (1997) Nat. Genet. 16:235.
Oda, T. et al. (1997) Genomics 43:376.
Entrez Gene IDs:
182 (Human); 29146 (Rat)
Alternate Names:
AGS; AHDMGC104644; Alagille syndrome; AWS; CD339 antigen; CD339; HJ1; JAG1; Jagged 1; Jagged1; JAGL1; protein jagged-1
