Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to block anti-TREM-1-induced TNF-alpha secretion by human peripheral blood mononuclear cells (PBMC). At 1 µg/mL, Recombinant Human TREM‑1 Fc Chimera is able to block > 70% of Goat Anti-Human TREM-1 (20 µg/mL, 200 µL/well) (Catalog # AF1278) induced TNF-alpha released from 1 x 10 5 cells.
Source
Mouse myeloma cell line, NS0-derived
Human TREM-1
(Ala21 - Arg200)
Accession # Q9NP99IEGRMD Human IgG1
(Pro100 - Lys330)N-terminus C-terminus Accession #
N-terminal Sequence
AnalysisAla21
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
47 kDa (monomer)
SDS-PAGE
60-70 kDa, reducing conditions
1278-TR |
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Formulation Lyophilized from a 0.2 μm filtered solution in PBS. | ||
Reconstitution Reconstitute at 100 μg/mL in sterile PBS. | ||
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | ||
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Background: TREM-1
TREM-1 (Triggering Receptor Expressed on Myeloid cells) is a type I transmembrane protein having a single Ig-like domain. It associates with the adapter protein, DAP12, to deliver an activating signal. Several other TREM family members have been reported that are structurally similar but share less than 30% amino acid identity. TREM-1 is expressed on blood neutrophils and a subset of monocytes, and expression is up-regulated by bacterial LPS. The natural ligand for TREM-1 has not been identified. However, engagement of TREM-1 with an agonist monoclonal antibody leads to expression of IL-8, MCP-1 and TNF-alpha, suggesting that this receptor plays an important role in inflammatory responses. TREM-1 is expressed at high levels on neutrophils of patients with microbial sepsis and in mice with LPS‑induced shock. Blockade of TREM-1 with a TREM‑1/Fc fusion protein protected mice against LPS‑induced shock. Human and mouse TREM-1 share approximately 42% amino acid sequence homology (1 - 3).
References:
Bouchon, A. (2000) J. Immunol. 164:4991.
Bouchon, A. (2001) Nature 410:1103.
Nathan, C. and A. Ding (2001) Nature Med. 7:530.
Long Name:
Triggering Receptor Expressed on Myeloid Cells 1
Entrez Gene IDs:
54210 (Human); 58217 (Mouse)
Alternate Names:
CD354 antigen; CD354; TREM1; TREM-1; TREM-1Triggering receptor expressed on monocytes 1; triggering receptor expressed on myeloid cells 1; triggering-receptor TREM1
