Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit Topflash reporter activity in HEK293T human embryonic kidney cells. The ED 50 for this effect is typically 150-900 ng/mL in the presence of 300 ng/mL of Recombinant Mouse Wnt‑3a (Catalog # ).
Source
Human embryonic kidney cell, HEK293-derived Ser32-Lys314
Accession #
N-terminal Sequence
AnalysisSer32
Predicted Molecular Mass
33 kDa
SDS-PAGE
37-43 kDa, reducing conditions
Carrier Free
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
9019-SF |
| 9019-SF/CF |
Formulation Lyophilized from a 0.2 μm filtered solution in NaH 2PO 4 and NaCl with BSA as a carrier protein. | Formulation Lyophilized from a 0.2 μm filtered solution in NaH 2PO 4 and NaCl. | |
Reconstitution Reconstitute at 500 μg/mL in PBS containing at least 0.1% human or bovine serum albumin. | Reconstitution Reconstitute at 500 μg/mL in PBS. | |
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | |
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
| Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: sFRP-1
Secreted Frizzled Related Protein-1 (sFRP-1), also known as SARP-2, FRP, and FrzA, belongs to a family of Wnt-binding proteins with homology to the ligand-binding domain of the Frizzled transmembrane Wnt receptors. The sFRP proteins are approximately 30-35 kDa in size and contain an N-terminal Frizzled-like domain with 10 conserved cysteines and a Netrin-like C-terminal domain (1-3). Mature mouse sFRP-1 shares 97% amino acid sequence identity with human and rat sFRP-1. It binds to Wnt-1, -2, -3a, and -4 and prevents their activation of canonical beta-catenin signaling (4-7). sFRP-1 is up-regulated in the ischemic heart where it limits inflammation and the severity of tissue damage following myocardial infarction (8-10). During angiogenesis it induces endothelial cell migration, tube formation, and vessel formation (11). sFRP-1 is expressed by bone marrow stromal cells and osteoclasts (12). It binds to TRANCE/RANK Ligand, inhibits osteoclast and osteoblast formation and survival, and inhibits the formation of trabecular bone (13, 14). sFRP-1 additionally restricts kidney tubule formation (7), the inflammatory activation of leukocytes (10), early B lymphoid progression (12), and vitreous fluid outflow in the eye (15).
References:
Cruciat, C.M. and C. Niehrs (2013) Cold Spring Harb. Perspect. Biol. 5:e015081.
van Amerongen, R. and R. Nusse (2009) Development 136:3205.
Rattner, A. et al. (1997) Proc. Natl. Acad. Sci. USA 94:2859.
Bafico, A. et al. (1999) J. Biol. Chem. 274:16180.
Dennis, S. et al. (1999) J. Cell Sci. 112:3815.
Wawrzak, D. et al. (2007) Biochem. Biophys. Res. Commun. 357:1119.
Yoshino, K. et al. (2001) Mech. Dev. 102:45.
Barandon, L. et al. (2002) Circulation 108:2282.
Sklepkiewicz, P. et al. (2015) Circ. Heart Fail. 8:362.
Barandon, L. et al. (2011) Arterioscler. Thromb. Vasc. Biol. 31:e80.
Dufourcq, P. et al. (2002) Circ. Res. 106:3097.
Yokota, T. et al. (2008) J. Immunol. 181:6061.
Hausler, K.D. et al. (2004) J. Bone Miner. Res. 19:1873.
Bodine, P.V.N. et al. (2004) Mol. Endocrinol. 18:1222.
Wang, W.-H. et al. (2008) J. Clin. Invest. 118:1056.
Long Name:
Secreted Frizzled Related Protein 1
Entrez Gene IDs:
6422 (Human); 20377 (Mouse); 84402 (Rat)
Alternate Names:
FRP1; FRP-1FrzA; FRPSARP-2; FrzA; SARP-2; SARP2sFRP-1; Secreted apoptosis-related protein 2; secreted frizzled-related protein 1; sFRP1; sFRP-1
