Purity
>85%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit the cell growth of DU145 human prostate carcinoma cells. Miyazaki, H. et al. (2004) Oncogene 23:9326. The ED 50 for this effect is 0.7-3.5 μg/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived Ala264-His402
Accession #
N-terminal Sequence
AnalysisAla264
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
16 kDa
SDS-PAGE
17-20 kDa, reducing conditions
9316-BP |
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Formulation Lyophilized from a 0.2 μm filtered solution in HCl. | ||
Reconstitution Reconstitute at 250 μg/mL in 4 mM HCl. | ||
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | ||
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Background: BMP-8b
BMP-8, also known as osteogenic protein 2 (OP-2), was first isolated from a hippocampal library in a screen to identify relatives of BMP-7/OP-1 (1). BMPs are a family of structurally and functionally related proteins and represent a subfamily of the transforming growth factor beta (TGF-beta ) superfamily. BMPs are involved in a wide range of processes including embryogenesis, tissue morphogenesis, cell differentiation and migration, and tumorigenesis. Cellular responses to BMPs are mediated by hetero-oligomeric complexes of type I and type II serine/threonine kinase receptors (2-4). BMP-8a and BMP-8b, produced from separate genes, share 98% aa sequence identity in human but only 74% in mouse within the mature regions. Human BMP-8b is synthesized as a 402 aa precursor protein that is cleaved between Arg263 and Ala264 to release the C-terminal mature protein. Mature human BMP-8b shares 91% and 71% aa sequence identity with mouse mature BMP-8a and -8b, respectively. BMP-8a is expressed during pregnancy in the deciduum and trophoblast cells, by inner root sheath cells of developing hair follicles, and by the epididymis and spermatids (5-7). In the mouse it cooperates with BMP-7 in the maintenance of spermatogenesis but is not required for the initiation of spermatogenesis (7, 8). BMP-8b, in contrast, is required for both the initiation and maintenance of spermatogenesis (9). BMP-8a is induced during osteoblast differentiation at the onset of mineralization and during the osteogenic phase of bone repair in osteoblasts and osteocytes (10-12). BMP-8b is also highly expressed in osteosarcomas and pancreatic cancer, and it contributes to tumor progression (13, 14). BMP-8b is also expressed in adipose tissue and the hypothalamus where it contributes to the regulation of energy balance and thermogenesis (15, 16).
References:
Ozkaynak, E. et al. (1992) J. Biol. Chem. 267:25220.
Chen, D. et al. (2004) Growth Factors 22:233.
Bragdon, B. et al. (2010) Cell Signal. Oct 16 epub.
Singh, A. and R.J. Morris (2010) Cytokine Growth Factor Rev. 21:299.
Zhao, G.-Q. and B.L. Hogan (1996) Mech. Dev. 57:159.
Ying, Y. and G.-Q. Zhao (2000) Biol. Reprod. 63:1781.
Zhao, G.-Q. et al. (1998) Development 125:1103.
Zhao, G.-Q. et al. (2001) Dev. Biol. 240:212.
Zhao, G.-Q. et al. (1998) Genes Dev. 10:1657.
van der Horst, G. et al. (2002) Bone 31:661.
Cho, T.-J. et al. (2002) J. Bone Miner. Res. 17:513.
Paic, F. et al. (2009) Bone 45:682.
Sulzbacher, I. et al. (2002) J. Clin. Pathol. 55:381.
Cheng, Z. et al. (2014) Oncol. Rep. 32:1861.
Whittle, A.J. et al. (2012) Cell 149:871.
Martins, L. et al. (2016) Cell Rep. 16:2231.
Long Name:
Bone Morphogenetic Protein 8b
Entrez Gene IDs:
656 (Human); 12164 (Mouse)
Alternate Names:
BMP-8; BMP-8b; bone morphogenetic protein 8b; MGC131757; OP-3
