Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by the ability of the immobilized protein to support the adhesion of the L Cells mouse fibroblast cell line. When 5 x 10 4 cells/well are added to Recombinant Mouse EpCAM/TROP-1 and Human Fibronectin (0.5 μg/mL, Catalog #) coated plates, cell adhesion is enhanced in a dose dependent manner. The ED 50 for this effect is 0.4-2.4 μg/mL
Source
Mouse myeloma cell line, NS0-derived
Mouse EpCAM/TROP1
(Gln24 & Arg81-Thr266)
Accession # Q99JW5IEGRMDP Mouse IgG2a
(Glu98-Lys330)N-terminus C-terminus Accession #
N-terminal Sequence
AnalysisArg81 detected. Gln24 inferred from enzymatic pyroglutamate treatment revealing Arg25
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
55 kDa
SDS-PAGE
58-75 kDa and 8 kDa
9364-EP |
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Formulation Lyophilized from a 0.2 μm filtered solution in PBS. | ||
Reconstitution Reconstitute at 500 μg/mL in PBS. | ||
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | ||
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Data Images
Bioactivity
| Immobilized Recombinant Mouse EpCAM/TROP1 (Catalog # 9364-EP)enhances L Cells mouse fibroblasts adhesion in the presence of Human Fibronectin (Catalog # 1). The ED50 for this effect is |
Background: EpCAM/TROP1
Epithelial Cellular Adhesion Molecule (EpCAM), also known as KS1/4, gp40, GA733-2, 17-1A, CD326 and TROP‑1, is a 40 kDa transmembrane glycoprotein composed of a 243 amino acid (aa) extracellular domain with two epidermal-growth-factor-like (EGF‑like) repeats, a 23 aa transmembrane domain, and a 26 aa cytoplasmic domain. Human and mouse EpCAM share 82% aa sequence identity. In mouse, EpCAM also shares 51% aa sequence homology with Trop‑2. EpCAM is detected in basolateral cell membranes of all simple epithelia and expression has been found to increase in epithelia tissues during fetal development (1, 2). EpCAM has been shown to function as a homophilic Ca2 + independent adhesion molecule, but it does not structurally resemble any of the four major families of cell adhesion molecules (3). Homophilic adhesion via EpCAM requires the interaction of both EGF‑like repeats, with the first EGF‑like repeat mediating reciprocal interaction between EpCAM molecules on opposing cells, while the second repeat is involved in lateral interaction of EpCAM (4). EpCAM has been identified as a surface marker for pluripotent embryonic stem cells and is strongly associated with the maintenance of the undifferentiated state of ESCs (5). EpCAM is highly expressed on the surface of epithelial cancer cells including colon, stomach, lung, pancreas, prostate and breast (6, 7).
References:
Balzar, M. et al. (1999) J. Mol. Med. 77:699.
Keisuke, N. et al. (2009) PLoS One 4 :e8543
Litvinov, S.V. et al. (1994) J. Cell Biol. 125:437.
Balzar, M. et al. (2001) Mol. Cell. Biol. 21:2570.
González, B. et al. (2009) Stem Cells, 27: 1782.
Went, P. et al. (2006) Br. J. Cancer 94:128.
Spizzo, G.S. et al. (2002) Int. J. Cancer 98:883.
Long Name:
Epithelial Cell Adhesion Molecule
Entrez Gene IDs:
4072 (Human); 17075 (Mouse)
Alternate Names:
17-1A; 323/A3; ACSTD1; antigen identified by monoclonal AUA1; CD326 antigen; CD326; Cell surface glycoprotein Trop-1; chromosome 4, surface marker (35kD glycoprotein); DIAR5; EGP; EGP-2; EGP314; EGP40; EpCAM; epithelial cell adhesion molecule; Epithelial cell surface antigen; Epithelial glycoprotein 314; Epithelial glycoprotein; ESA; GA733-2; GA733-2EGP34; gp40; hEGP314; HNPCC8; KS 1/4 antigen; KS1/4; KSAHEA125; M1S2; M4S1; M4S1Ly74; Major gastrointestinal tumor-associated protein GA733-2; MIC18MH99; MOC31; TACST-1; TACSTD1; TROP1; TROP1CD326; Tumor-associated calcium signal transducer 1CO-17A
