Purity
>85%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Human C1qL3 (Catalog # ) is immobilized at 2 μg/mL, 100 μL/well, the concentration of Recombinant Human BAI2 Fc Chimera that produces 50% of the optimal binding response is approximately 25-150 ng/mL.
Source
Human embryonic kidney cell, HEK293-derived
Human BAI2
(Phe21-Asp911 )
Accession # O60241-3IEGRMD Human IgG1
(Pro100-Lys330)N-terminus C-terminus Accession #
N-terminal Sequence
AnalysisPhe21
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
123 kDa
SDS-PAGE
128-200 kDa, reducing conditions
9338-BA |
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Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. | ||
Reconstitution Reconstitute at 250 μg/mL in PBS. | ||
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | ||
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Data Images
Bioactivity
| WhenRecombinant Human C1qL3 (Catalog # ) is coated at 2 µg/mL (100 μL/well), Recombinant Human BAI2 FcChimera (Catalog # 9338-BA) binds with an ED50 of 25-150 ng/mL. |
Background: BAI2
Brain angiogenesis inhibitor 2 (BAI2), along with BAI1 and BAI3, form a subfamily of brain specific adhesion GPCRs. They are large, glycosylated molecules, approximately 180-220 kDa, and play a role in neuronal synapse formation and maintenance. Mature human BAI2 consists of a 903 amino acid (aa) N-terminal extracellular domain (ECD) containing four TSP1 domains, a hormone binding domain (HBD), a GAIN domain and a GPS protease sensitive linker, followed by a region with seven transmembrane segments and a 410 aa C-terminal cytoplasmic domain (1). Within the N-terminal ECD, human BAI2 shares 96% aa sequence identity with both mouse and rat BAI2. BAI2 is expressed primarily in neurons and astrocytes of the hippocampus and cerebral cortex in the brain (2, 3). To become functional, BAI2 might require activation by cleavage at the GPS domain (4). BAI family members are known to bind the C1q-like complement (C1qL) family via thrombospondin type 1 repeats (TSRs) which are conserved between BAI members (5). BAI2 has been shown to suppress expression of VEGF leading to increased neurogenesis in the dentate gyrus of the hippocampus (6).
References:
Duman, J.G. et al. (2016) Neural Plast. 2016:8301737.
Shiratsuchi, T. et al. (1997) Cytogenet. Cell Genet. 79:103.
Kee, H.J. et al. (2002) J. Cereb. Blood Flow Metab. 22:1054.
Daisuke, O. et al. (2010) J. Recept Signal Transduct Res 30:143.
Bolliger, M.F. et al. (2011) Proc. Natl. Acad. Sci. USA 108:2534.
Jeong, B.C. et al. (2016) FEBS Lett 580:669.
Long Name:
Brain-specific Angiogenesis Inhibitor 2
Entrez Gene IDs:
576 (Human); 230775 (Mouse); 313058 (Rat)
Alternate Names:
BAI2; brain-specific angiogenesis inhibitor 2; Brain-specific angiongenesis inhibitor-2
