Purity
>85%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by the ability of the immobilized protein to support the adhesion of CCD-1070Sk human normal skin fibroblasts. The ED 50 for this effect is 0.1-0.4 μg/mL
Source
Chinese Hamster Ovary cell line, CHO-derived Arg23-Lys1403, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
AnalysisLeu28
Predicted Molecular Mass
150 kDa
SDS-PAGE
135-230 kDa, reducing conditions
9335-SN |
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Formulation Lyophilized from a 0.2 μm filtered solution in PBS. | ||
Reconstitution Reconstitute at 500 μg/mL in PBS. | ||
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | ||
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Data Images
Bioactivity
| Immobilized Recombinant Mouse SNED1 (Catalog #9335-SN) supports the adhesion of CCD-1070Sk human normal skinfibroblasts The ED50 for this effect is 0.1-0.4 μg/mL. |
Background: SNED1
SNED1 (Sushi, nidogen and EGF-like domain-containing protein 1), also known as stromal nidogen extracellular matrix protein (Snep) and secreted protein SST-3, is an approximately 150 kDa protein (1). Mature mouse SNED1 contains 1379 aa that includes a Sushi/CCP (complement control protein) domain, an NIDO domain, 15 EGF-like domains, and 3 fibronectin type-III domains (1). Mouse SNED1 shares 84% and 96% aa sequence identity with human and rat SNED1, respectively. Alternative splicing generates an isoform lacking the Sushi/CCP domain, normally found in the center of the protein. SNED1 is mainly expressed in kidney stromal cells, but can be found in mesenchymal cells of other embryonic tissues, and within the nervous system (1,2). SNED1 has been found to have a role in tumor progression and metastasis. Studies show that SNED1 is up-regulated in highly metastatic mammary tumors while it is found to have relatively lower expression levels in poorly metastatic mammary tumors (3). SNED1 has also been shown to be up-regulated in pancreatic ductal adenocarcinoma cells which correlates with an increased chemoresistance (4). Additionally, SNED1 is found to be expressed specifically in stroma cells of the kidney and other organs in areas that undergo apoptosis during embryonic development making it important for kidney development (1).
References:
Leimeister, C. et al. (2004) Developmental Dynamics. 230:371.
Ueno, H. et al. (2003) Nature Immunology. 4:457.
Naba, A. et al. (2014) eLife. 3:e01308.
Longati, P. et al. (2013) BMC Cancer. 13:95.
Long Name:
Sushi, Nidogen and EGF-like Domain-containing Protein
Entrez Gene IDs:
25992 (Human); 208777 (Mouse); 316638 (Rat)
Alternate Names:
DKFZp586B2420; FLJ00133,6720455I24Rik homolog; insulin responsive sequence DNA binding protein-1; Insulin-responsive sequence DNA-binding protein 1; IRE-BP1; SNED1; Snep; SST3; sushi, nidogen and EGF-like domain-containing protein 1; sushi, nidogen and EGF-like domains 1
