Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to induce adhesion of ATDC5 mouse chondrogenic cells. The ED 50 for this effect is 0.9-4.5 μg/mL.
Source
Human embryonic kidney cell, HEK293-derived Gln35-His424, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
AnalysisNo results obtained. Gln35 inferred from enzymatic pyroglutamate treatment revealing Val36
Predicted Molecular Mass
45 kDa
SDS-PAGE
62-73 kDa, reducing conditions
9318-P6 |
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Formulation Lyophilized from a 0.2 μm filtered solution in PBS. | ||
Reconstitution Reconstitute at 500 μg/mL in PBS. | ||
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | ||
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Data Images
Binding Activity
| Recombinant Human PSG-6 (Catalog # 9318-P6) induces adhesion of ATDC5 mouse chondrogenic cells. The ED50 for this effect is 0.9-4.5 μg/mL. |
Background: PSG-6
Pregnancy-specific glycoprotein 6 (PSG-6), also known as PSG10 and PSGGB is a secreted glycoprotein of the PSG family within the CEA (carcinoembryonic antigen) superfamily (1). Mature human PSG-6 is a 401 amino acid (aa) protein that consists of four tandem immunoglobulin-like domains followed by a 30 aa
C-terminal tail (2, 3). The N-terminal Ig-like domain contains an Arg-Gly-Asp (RGD) sequence which is a consensus integrin-binding motif found in many adhesion proteins (4, 5). Alternative splicing generates an additional isoform with a substitution in the C-terminal tail. PSG-6 is predominantly expressed in placenta and more weakly in granulocytes (3). It induces monocyte production of the cytokines IL-6, IL-10, and TGF-beta 1 (6).
References:
Kuespert, K. et al. (2006) Curr. Opin. Cell Biol. 18:565.
Zimmermann, W. et al. (1989) Biochem. Biophys. Res. Commun. 163:1197.
Barnett, T.R. et al. (1990) Biochemistry 29:10213.
Leslie, K.K. et al. (1990) Proc. Natl. Acad. Sci. USA 87:5822.
Ruoslahti, E. (1996) Annu. Rev. Cell Dev. Biol. 12:697.
Snyder, S.K. et al. (2001) Am. J. Reprod. Immunol. 45:205.
Long Name:
Pregnancy-specific beta-1-glycoprotein 6
Entrez Gene IDs:
5675 (Human)
Alternate Names:
CGM3; pregnancy specific beta-1-glycoprotein 6; Pregnancy-specific beta-1-glycoprotein 10; Pregnancy-specific beta-1-glycoprotein 12; pregnancy-specific beta-1-glycoprotein 6; Pregnancy-specific glycoprotein 10; Pregnancy-specific glycoprotein 12; Pregnancy-specific glycoprotein 6; PS-beta-G-10; PS-beta-G-12; PS-beta-G-6; PSBG-10; PSBG-12; PSBG-6; PSG10; PSG12; PSG6; PSG-6; PSGGB
