Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Neu5Ac(a2-6)GalNAc-PAA-biotin is immobilized onto a Streptavidin-coated plate, Recombinant Human Siglec-15 Fc Chimera binds with an ED 50 of 150-900 ng/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived
Human Siglec-15
(Phe20-Thr263)
Accession # Q6ZMC9IEGRMD Human IgG1
(Pro100-Lys330)N-terminus C-terminus Accession #
N-terminal Sequence
AnalysisPhe20
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
53 kDa
SDS-PAGE
53-64 kDa, reducing conditions
9227-SL |
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Formulation Lyophilized from a 0.2 μm filtered solution in PBS, NaCl, CHAPS and Trehalose. | ||
Reconstitution Reconstitute at 200 μg/mL in water. | ||
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | ||
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Data Images
Bioactivity
| When Neu5Ac(a2-6)GalNAc-PAA-biotin is immobilized onto aStreptavidin-coated plate, Recombinant Human Siglec-15 Fc Chimera (Catalog # 9227-SL) bindswith an ED50 of 150-900 ng/mL. |
Background: Siglec-15
Siglec-15 is a transmembrane glycoprotein in the Siglec family of sialic acid-binding immune regulatory molecules (1). Mature human Siglec-15 consists of a 244 amino acid (aa) extracellular domain (ECD) with two Ig-like domains, a 21 aa transmembrane segment, and a 44 aa cytoplasmic domain (2). Within the ECD, human Siglec-15 shares 85% aa sequence identity with mouse and rat Siglec-15. Alternative splicing generates an additional isoform that lacks the signal peptide and first Ig-like domain. Siglec-15 associates with the signaling molecules DAP12 and DAP10 (2-5). It is expressed on osteoclasts, macrophages, and dendritic cells (2-6) and binds to the sialyl-Tn antigen (2, 3, 6). This interaction induces the production of TGF-beta by tumor-associated macrophages (3). Siglec-15 function is important for osteoclast formation and TRANCE/RANK Ligand signaling in osteoclasts (4-6).
References:
Humphrey, M.B. and M.C. Nakamura (2015) Clin. Rev. Allergy Immunol. PMID 26573914.
Angata, T. et al. (2007) Glycobiology 17:838.
Takamiya, R. et al. (2013) Glycobiology 23:178.
Kameda, Y. et al. (2013) J. Bone Miner. Res. 28:2463.
Stuible, M. et al. (2014) J. Biol. Chem. 289:6498.
Ishida-Kitagawa, N. et al. (2012) J. Biol. Chem. 287:17493.
Long Name:
Sialic Acid Binding Ig-like Lectin 15
Entrez Gene IDs:
284266 (Human); 620235 (Mouse); 498888 (Rat)
Alternate Names:
CD33 antigen-like 3SIGLEC-15; CD33 molecule-like 3; CD33L3; HsT1361; sialic acid binding Ig-like lectin 15; sialic acid-binding Ig-like lectin 15; Siglec15; Siglec-15
