Purity
>80%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by the ability of the immobilized protein to support the adhesion of A431 human epithelial carcinoma cells. The ED 50 for this effect is 0.4-2.4 μg/mL.
Source
Human embryonic kidney cell, HEK293-derived
Human Desmoglein-4
(Glu24-Ala631)
Accession # Q86SJ6IEGRMD Human IgG1
(Pro100-Lys330)N-terminus C-terminus Accession #
N-terminal Sequence
AnalysisGlu24
Structure / Form
Disulfide-liked homodimer
Predicted Molecular Mass
94 kDa
SDS-PAGE
95-115 kDa, reducing conditions
9230-DM |
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Formulation Lyophilized from a 0.2 μm filtered solution in PBS. | ||
Reconstitution Reconstitute at 500 μg/mL in PBS. | ||
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | ||
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Data Images
Bioactivity
| Immobilized Recombinant Human Desmoglein‑4 Fc Chimera (Catalog # 9230-DM) supports the adhesion of A431 human epithelial carcinoma cells. The ED50 for this effect is 0.4-2.4 μg/mL. |
Background: Desmoglein-4
Desmoglein-4 is an approximately 140 kDa transmembrane glycoprotein in the cadherin family of calcium dependent adhesion molecules. Human Desmoglein-4 is synthesized with a 26 amino acid (aa) propeptide followed by a 582 aa extracellular domain (ECD) with four tandem cadherin-like domains, a 21 aa transmembrane segment, and a 388 aa cytoplasmic domain (1, 2). Within the ECD, human Desmoglein-4 shares 82% aa sequence identity with mouse and rat Desmoglein-4. Alternative splicing generates additional isoform with a substitution in the cytoplasmic domain. Desmoglein-4 is the principal cadherin of desmosomes which form adhesive contacts between epithelial cells (2, 3). It is expressed in hair follicles and differentiated keratinocytes in suprabasal layers of the skin and plays a role in keratinocyte differentiation and adhesion (2, 3). Mutations in human Desmoglein-4 are associated with the development of monilethrix autosomal recessive hypotrichosis (LAH) (2, 4-8). Autoantibodies directed against Desmoglein-3 in the blistering skin disorder pemphigus vulgaris can cross-react with Desmoglein-4 (2, 9, 10).
References:
Whittock, N.V. and C. Bower (2003) J. Invest. Dermatol. 120:523.
Kljuic, A. et al. (2003) Cell 113:249.
Bazzi, H. et al. (2006) Differentiation 74:129.
Bazzi, H. et al. (2005) J. Investig. Dermatol. Symp. Proc. 10:222.
Schaffer, J.V. et al. (2006) J. Invest. Dermatol. 126:1286.
Shimomura, Y. et al. (2006) J. Invest. Dermatol. 126:1281.
Zlotogorski, A. et al. (2006) J. Invest. Dermatol. 126:1292.
Kato, M. et al. (2015) J. Invest. Dermatol. 135:1253.
Nagasaka, T. et al. (2004) J. Clin. Invest. 114:1484.
Calkins, C.C. et al. (2006) J. Biol. Chem. 281:7623.
Entrez Gene IDs:
147409 (Human); 16769 (Mouse); 291754 (Rat)
Alternate Names:
Cad13; Cadherin family member 13; CDGF13; CDHF13; CDHF13CDH family member 13; desmoglein 4; Desmoglein4; Desmoglein-4; DSG4; LAHdesmoglein-4
