Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Human IL-17RC Isoform 1 Fc Chimera (Catalog # 9284-IL) is coated at 0.5 µg/mL, Recombinant Human IL-17/IL-17A (Catalog # ) binds with an ED 50 = 0.1-0.6 ng/mL.
Source
Human embryonic kidney cell, HEK293-derived
Human IL-17 RC Isoform 1
(Leu21-His465)
Accession # NP_703190.1IEGRMD Human IgG1
(Pro100-Lys330)N-terminus C-terminus Accession #
N-terminal Sequence
AnalysisLeu21
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
76 kDa
SDS-PAGE
95-113 kDa, reducing conditions
9284-IL |
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Formulation Lyophilized from a 0.2 μm filtered solution in PBS. | ||
Reconstitution Reconstitute at 500 μg/mL in PBS. | ||
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | ||
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Data Images
Bioactivity
| When Recombinant Human IL-17 RC Isoform 1 Fc Chimera (Catalog # 9284-IL) is coated at 0.5 µg/mL, Recombinant Human IL-17/IL-17A (Catalog # ) binds with an ED50 = 0.1-0.6 ng/mL. |
Background: IL-17 RC
IL-17 receptor C (IL-17RC; also known as IL-17RL and IL-17Rhom) is an 85-110 kDa member of the IL-17 receptor family. IL-17 plays a key role in the development of autoimmunity and inflammation (1, 2). Human IL-17 RC is a type I transmembrane glycoprotein that is expressed on neutrophils, endothelial cells, chondrocytes and osteoblasts, breast and prostatic epithelium, fibroblasts, renal tubular epithelium, and skeletal muscle cells (3-5). Mature human IL-17 RC consists of a 518 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane segment, and a 232 aa cytoplasmic region (6). It is estimated that there are over 90 alternative splice forms, with transmembrane-containing isoforms predominating (4, 7). This recombinant protein corresponds to IL-17 RC isoform 1, the canonical form. Within the ECD, IL-17 RC isoform 1 shares 69% aa sequence identity with mouse IL-17 RC. IL-17 RC is the cognate receptor for IL-17F, and it binds IL-17A with similar affinity (8). IL-17 RC associates wiith IL-17 RA to form a receptor complex for both IL-17A and IL-17F (8-11).
References:
Song, X. and Y. Qian (2013) Cytokine 62:175.
Weaver, C.T. et al. (2007) Annu. Rev. Immunol. 25:821.
Taylor, P.R. et al. (2014) Nat. Immunol. 15:143.
You, Z. et al. (2007) Neoplasia 9:464.
Kokubu, T. et al. (2008) J. Hictochem. Cytochem. 56:89.
Haudenschild, D. et al. (2002) J. Biol. Chem. 277:4309.
Haudenschild, D.R. et al. (2006) Prostate 66:1268.
Kuestner, R.E. et al. (2007) J. Immunol. 179:5462.
Toy, D. et al. (2006) J. Immunol. 177:36.
Kramer, J.M. et al. (2006) J. Immunol. 176:711.
Kramer, J.M. et al. (2007) J. Immunol. 179:6379.
Long Name:
Interleukin 17 Receptor C, Transcript Variant 3
Entrez Gene IDs:
84818 (Human); 171095 (Mouse)
Alternate Names:
FLJ95963; FLJ96005; IL-17 RC; IL-17 receptor C; IL-17 RL; IL17F receptor; IL17RC; IL-17RC; IL17Rhom; IL17RL; IL-17RL; IL17-RL; interleukin 17 receptor C; interleukin-17 receptor C; Interleukin-17 receptor homolog; Interleukin-17 receptor-like protein; MGC10763; zcytoR14
