Kit Summary
For the differentiation of regulatory T Cells (Tregs) from a preparation of naïve CD4+ T cells.
Key Benefits
Contains high quality bioactive proteins
Yields a highly enriched population of FoxP3+CD25+ Treg cells
Provides optimized reagents for inducing Tregs from CD4+ T cells
Involves validated and straightforward procedures
Does not require specialized instrumentation
Why Differentiate Treg Cells In Vitro?
Forkhead Box P3 (FoxP3)+ regulatory T (Treg) cells are a suppressive subset of CD4+ T cells that function to antagonize immune responses. Treg cells have the capacity to prevent potentially damaging autoimmune and protective immune responses, so the number of Treg cells is a crucial determinant of the regulatory burden on the immune system. In vitro differentiation of Treg cells from the larger naïve CD4+ T cell population provides increased numbers of Treg cells to facilitate downstream research.
Kit Contents
This kit contains the following optimized proteins and reagents to drive efficient differentiation of naïve CD4+ T cells into FoxP3+CD25+ Treg cells.
Hamster Anti-Mouse CD3 Antibody
Mouse Treg Reagent 1
Mouse Treg Reagent 2
Mouse Treg Reagent 3
Reconstitution Buffer 1
Reconstitution Buffer 2
Wash Buffer (20X)
Stability and Storage
Store the unopened kit at -20 °C. After opening the kit, the Human Anti-Mouse CD3 Antibody, Mouse Treg Reagent 1, and Mouse Treg Reagent 2 may be stored at 2-8 °C under sterile conditions for up to 30 days or at -20 °C to -70 °C in a manual defrost freezer for up to 3 months. Mouse Treg Reagent 3 may be stored at -20 °C to -70 °C in a manual defrost freezer for up to 3 months. Reconstitution Buffer 1, Reconstitution Buffer 2, and 20X Wash Buffer may be stored under sterile conditions for up to 3 months at 2 to 8 °C. Storage conditions are valid if used within the expiration date of the kit.
Data Examples
Differentiation of CD4+ T Cells into Treg Cells Confirmed by FoxP3 and CD25 Expression. Naïve CD4+ T cells were incubated without (A, B) or with (C, D) reagents included in the CellXVivo™ Mouse Treg Cell Differentiation Kit for 5 days. Cells were fixed, permeabilized, and stained using the FlowX Mouse Regulatory T Cell Kit (Catalog # ). Cells were imaged using flow cytometry. Quadrants were set based on isotope-stained samples. |
Preparation and Storage
Stability & Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
