Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by the ability of the immobilized protein to support the adhesion of ICAM-3 expressing CHO Chinese hamster ovary cells. When 5 x 10 4 cells/well are added to Recombinant Human DC‑SIGN/CD209 Fc Chimera coated plates (5 µg/mL with 100 µL/well), approximately 50-80% of added cells will adhere after 1 hour at room temperature.
Optimal dilutions should be determined by each laboratory for each application.Source
Mouse myeloma cell line, NS0-derived
MD Human IgG1
(Pro100-Lys330)IEGR Human DC-SIGN
(Lys62-Ala404)
Accession # Q9NNX6N-terminus C-terminus Accession #
N-terminal Sequence
AnalysisMet
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
65.7 kDa (monomer)
SDS-PAGE
69 kDa, reducing conditions
161-DC |
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Formulation Lyophilized from a 0.2 μm filtered solution in PBS. | ||
Reconstitution Reconstitute at 100 μg/mL in sterile PBS. | ||
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | ||
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Background: DC-SIGN/CD209
Human Dendritic Cell-specific ICAM-3 Grabbing Non-integrin (DC-SIGN)/CD209 is a member of the C-type lectin family (1). The canonical DC-SIGN/CD209 isoform is a 46 kDa, 404 amino acid (aa) type II transmembrane protein (2). The extracellular region contains a Ca 2+-dependent carbohydrate-binding lectin domain (2). Multiple human DC-SIGN/CD209 splice forms exist, generating both membrane-bound and soluble forms (3). DC-SIGN/CD209 is not well conserved between mouse and human, with the extracellular domain sharing only 63% aa identity. The DC-SIGN/CD209 lectin domain binds mannose oligosaccharides on pathogens including HIV as well as self glycoproteins including ICAMs (2, 4). DC-SIGN/CD209 is expressed on dendritic cells (DC) and inflammatory macrophages and contributes to antigen presentation (5, 6).
References:
Liu, W. et al. (2004) J. Biol. Chem. 279:18748.
Curtis, B.M. et al. (1992) Proc. Natl. Acad. Sci. USA 89:8356.
Mummidi, S. et al. (2001) J. Biol. Chem. 276:33196.
Anthony, R.M. et al. (2008) Proc. Natl. Acad. Sci. USA 105:19571.
Geijtenbeek, T.B. et al. (2000) Cell 100:575.
Garcia-Vallejo, J.J. and Y. van Kooyk (2013) Trends Immunol. 34:482.
Long Name:
Dendritic Cell-specific ICAM-3-grabbing Non-integrin 1
Entrez Gene IDs:
30835 (Human); 170786 (Mouse)
Alternate Names:
CD209 antigendendritic cell-specific intracellular adhesion molecules (ICAM)-3 grabbingnon-integrin; CD209 molecule; CD209; CDSIGNHIV gpl20-binding protein; CLEC4L; CLEC4LC-type lectin domain family 4 member L; DCSIGN; DC-SIGN; DC-SIGN1; DC-SIGN1C-type lectin domain family 4, member L; DC-SIGNMGC129965; Dendritic cell-specific ICAM-3-grabbing non-integrin 1
