Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Human SLITRK5 (Catalog # is coated at 500 ng/mL, Recombinant Human PSG-1 (Catalog # 9) binds with an ED 50 = 50-300 ng/mL.
Source
Mouse myeloma cell line, NS0-derived Gln35-Pro419, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
AnalysisGln35 & Val36
Predicted Molecular Mass
44 kDa
SDS-PAGE
64-77 kDa, reducing conditions
9334-P1 |
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Formulation Lyophilized from a 0.2 μm filtered solution in PBS with trehalose. | ||
Reconstitution Reconstitute at 500 μg/mL in PBS. | ||
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | ||
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Data Images
Bioactivity
| WhenRecombinant Human SLITRK5 (Catalog # ) is coated at 500 ng/mL,Recombinant Human PSG-1 (Catalog # 9334-P1) binds with an ED50 = 50-300 ng/mL. |
Background: PSG-1
Pregnancy-specific beta-1-glycoprotein 1 (PSG-1), also called SP1, PSG1 or B1G1 and designated CD66f, is a secreted glycoprotein of the human PSG family within the CEA (carcinoembryonic antigen) superfamily (1-3). PSG-1 shares 84-91% amino acid (aa) sequence identity with the most similar family members, PSG-3, -4, -6, -7, and -8. Mature PSG-1 contains a V-type Ig-like domain that is important for adhesion (N1), and three C2-type Ig-like domains (A1, A2 and B2). Three potential isoforms vary at the extreme C-terminus. Although rodents express pregnancy-specific glycoproteins, identity with human PSG proteins is low and limited to the N1 domain (3). PSG1 is one of the most abundant fetal proteins found in maternal blood during pregnancy. It is secreted by syncytiotrophoblasts and exerts proangiogenic effect by inducing VEGF-A secretion and formation of tubes in endothelial cells (4). PSG-1 also serves an immunomodulatory function through
up-regulation of TGF-beta in macrophages, monocytes, and trophoblasts (5). In addition PSG-1 has been shown to induce secretion of anti-inflammatory cytokines
IL-10 and IL-6 in human monocytes (6). PSG-1 binds Integrin alpha IIb beta 3 and inhibits platelet-fibrinogen interactions (7). Recent studies also suggest PSG-1's involvement in cancer biology and correlation with chemotherapy resistance in breast cancer (8).
References:
Watanabe, S. and J.Y. Chou (1988) J. Biol. Chem. 263:2049.
Leslie, K.K. et al. (1990) Proc. Natl. Acad. Sci. USA 87:5822.
McLellan, A.S. et al. (2005) BMC Evol. Biol. 5:39.
Lisboa, F. et al. (2011) J Biol Chem. 286:7577.
Ha, C. et al. (2010) Biol Rep. 83:27.
Snyder, S. et al. (2001) Am J Reprod Immunol. 45:205.
Shanley D. et al. (2013) PLoS One. 8:e57491.
He, D. et al. (2016) Clin Sci (Lond). 130:2267.
Long Name:
Pregnancy-specific beta-1-glycoprotein 1
Entrez Gene IDs:
5669 (Human)
Alternate Names:
B1G1; B1G1CD66 antigen-like family member F; CD66f antigen; CD66f; DHFRP2; Fetal liver non-specific cross-reactive antigen 1/2; FLJ90598; FLJ90654; FL-NCA-1/2; PBG1; pregnancy specific beta-1-glycoprotein 1; pregnancy-specific B-1 glycoprotein; Pregnancy-specific beta-1 glycoprotein C/D; pregnancy-specific beta-1-glycoprotein 1; Pregnancy-specific glycoprotein 1; PS-beta-C/D; PS-beta-G-1; PSbG1; PSBG-1; PSBG1SP1; PSG1; PSG-1; PSG95; PSGGA; PSGGAPSG95; PSGIIA
