Human P-Cadherin Alexa Fluor 488 MAb (Clone 104805) 100 TESTS

• Species Reactivity

  Human

• Specificity

  Detects human P-Cadherin in direct ELISAs and Western blots. In Western blots, does not cross-react with recombinant human (rh) Cadherin‑8, recombinant mouse P-Cadherin, or rhVE-Cadherin.

• Source

  Monoclonal Mouse IgG    ₁ Clone # 104805

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant human P-Cadherin    
  Asp108-Gly654    
  Accession # CAA45177

• Formulation

  Supplied in a saline solution containing BSA and Sodium Azide.

• Label

  Alexa Fluor 488

Applications

• Recommended    
  Concentration

  Sample

• Flow Cytometry

  5 µL/10    ⁶ cells

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Shipping

  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Protect from light.    Do not freeze.    

• 12 months from date of receipt, 2 to 8 °C as supplied.

Background: P-Cadherin

Placental (P) - Cadherin (PCAD) is a member of the Cadherin family of cell adhesion molecules. Cadherins are calcium-dependent transmembrane proteins, which bind to one another in a homophilic manner. On their cytoplasmic side, they associate with the three catenins, alpha, beta, and gamma (plakoglobin). This association links the cadherin protein to the cytoskeleton. Without association with the catenins, the cadherins are non-adhesive. Cadherins play a role in development, specifically in tissue formation. They may also help to maintain tissue architecture in the adult. P-Cadherin is a classical cadherin molecule. Classical cadherins consist of a large extracellular domain which contains DXD and DXNDN repeats responsible for mediating calcium-dependent adhesion, a single-pass transmembrane domain, and a short carboxy-terminal cytoplasmic domain responsible for interacting with the catenins. Human P-Cadherin is an 829 amino acid (aa) protein with a 26 aa signal sequence and an 803 aa propeptide. The mature protein begins at aa 108 and has a 548 aa extracellular region, a 23 aa transmembrane region, and a 151 aa cytoplasmic region. The human and mouse mature PCAD proteins share 87% homology.

• References:

1. Shimoyama, Y. et al. (1989) J. Cell Biol. 109:1787.

2. Bussemakers, M.J.G. et al. (1993) Mol. Biol. Reports 17:123.

3. Overduin, M. et al. (1995) Science 267:386.

4. Takeichi, M. (1991) Science 251:1451.

5. Nose, A. et al. (1987) EMBO J. 6:3655.

• Long Name:

  Placental Cadherin

• Entrez Gene IDs:

  1001 (Human); 12560 (Mouse)

• Alternate Names:

  CAD3; cadherin 3, P-cadherin (placental); cadherin 3, type 1, P-cadherin (placental); Cadherin-3; CDH3; CDHP; CDHPcalcium-dependent adhesion protein, placental; HJMD; PCAD; PCadherin; P-Cadherin; PCADP-cadherin; Placental cadherin