Species Reactivity
Human
Specificity
Detects human NKG2D/CD314 in Western blots.
Source
Monoclonal Mouse IgG 1 Clone # 149810
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
BaF3 mouse pro-B cell line transfected with human NKG2D/CD314
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin
Applications
Recommended
ConcentrationSample
Flow Cytometry
10 µL/10 6 cells
See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. are available in the Technical Information section on our website.
Data Examples
Flow Cytometry | Detection of NKG2D/CD314 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) gated on CD3- cells were stained with Mouse Anti-Human NCAM‑1/CD56 PE‑conjugated Monoclonal Antibody (Catalog # ) and either (A) Mouse Anti-Human NKG2D/CD314 APC‑conjugated Monoclonal Antibody (Catalog # FAB139A) or (B) Mouse IgG1 APC Isotype Control (Catalog # ). View our protocol for . |
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Background: NKG2D/CD314
NKG2D is a type II transmembrane glycoprotein having an extracellular lectin-like domain. This domain lacks the recognizable calcium-binding sites found in true C‑type lectins and binds protein rather than carbohydrate ligands. Human NKG2D is expressed on CD8+ alpha beta T cells, gamma δ T cells, NK cells, and NKT cells. In mouse systems NKG2D also occurs on macrophages. Human ligands for NKG2D include MICA, MICB, and ULBP1, 2, and 3. Expression of NKG2D ligands occurs in epithelial cells, tumor cells and under conditions of stress or infection. NKG2D exists as a disulfide-linked homodimer that delivers an activating signal upon ligand binding. Signaling requires association with an adapter protein. Alternative splicing of the NKG2D mRNA results in isoforms with different cytoplasmic domains that can associate either with DAP12 to deliver a true activating signal or with DAP10 resulting in a costimulatory signal. NKG2D has been implicated in anti-tumor surveillance and the immune response against viral infection.
References:
Li, P. et al. (2001) Nature Immunol. 2:443.
Steinle, A. et al. (2001) Immunogenetics 53:279.
Cosman, D. et al. (2001) Immunity 14:123.
Cerwenka, A. and L. Lanier (2001) Immunol. Rev. 181:158.
Wu, J. et al. (1999) Science 285:730.
Diefenbach, A. et al. (2002) Nature Immunol. 3:1142.
Gilfillan, S. et al. (2002) Nature Immunol. 3:1150.
Groh, V. et al. (2001) Nature Immunol. 2:255.
Cerwenka, A. et al. (2001) Proc. Natl. Acad. Sci. USA 98:11521.
Diefenbach, A. et al. (2001) Nature 413:165.
NKG2D and its Ligands (2002) .
Long Name:
Natural Killer G2D
Entrez Gene IDs:
22914 (Human); 27007 (Mouse)
Alternate Names:
CD314 antigen; CD314; D12S2489E; FLJ17759; FLJ75772; Killer cell lectin-like receptor subfamily K member 1; killer cell lectin-like receptor subfamily K, member 1; KLR; KLRK1; NK cell receptor D; NKG2-D type II integral membrane protein; NKG2D; NKG2-D; NKG2-D-activating NK receptor; NKG2DDNA segment on chromosome 12 (unique) 2489 expressed sequence
