Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated mouse T cells. The ED 50 for this effect is 0.15-0.75 µg/mL.
Source
Mouse myeloma cell line, NS0-derived
Mouse PD-L1
(Phe19-Thr238)
Accession # Q9EP73IEGRMD Human IgG1
(Pro100-Lys330)N-terminus C-terminus Accession #
N-terminal Sequence
AnalysisPhe19
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
51.3 kDa (monomer)
SDS-PAGE
75-85 kDa, reducing conditions
1019-B7 |
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Formulation Lyophilized from a 0.2 μm filtered solution in PBS. | ||
Reconstitution Reconstitute at 100 μg/mL in sterile PBS. | ||
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | ||
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Data Images
Bioactivity
| Recombinant Mouse PD-L1/B7-H1 Fc Chimera (Catalog # 1019-B7) inhibits anti-CD3-induced cell proliferation of stimulated mouse T cells. The ED50 for this effect is 0.15-0.75 μg/mL. |
SDS-PAGE
| 1 μg/lane of Recombinant Mouse PD‑L1/B7‑H1 Fc Chimera was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing bands at 79 kDa and 150 kDa, respectively. |
Background: PD-L1/B7-H1
B7-H1, also known as PD-L1 and CD274, is an approximately 65 kDa transmembrane glycoprotein in the B7 family of immune regulatory molecules (1). Mature mouse B7-H1 consists of a 221 amino acid (aa) extracellular domain (ECD) with two immunoglobulin-like domains, a 21 aa transmembrane segment, and a 30 aa cytoplasmic domain (2). Within the ECD, mouse B7-H1 shares 73% and 86% aa sequence identity with human and rat B7-H1, respectively. B7-H1 is expressed on inflammatory-activated immune cells including macrophages, T cells, and B cells (2-5), keratinocytes (6, 7), enothelial and intestinal epithelial cells (6, 8), as well as a variety of carcinomas and melanoma (9, 10). B7-H1 binds to T cell B7-1/CD80 and PD-1 (5, 6, 10-13). It suppresses T cell activation and proliferation (3, 6, 12, 14) and induces the apoptosis of activated T cells (9). It plays a role in the development of immune tolerance by promoting T cell anergy (5, 12) and enhancing regulatory T cell development (14). B7-H1 favors the development of anti-inflammatory IL-10 and IL-22 producing dendritic cells (3, 8) and inhibits the development of Th17 cells (14). In cancer, B7-H1 provides resistance to T cell mediated lysis, enhances EMT, and enhances the tumorigenic function of Th22 cells (4, 7, 10, 13).
References:
Ceeraz, S. et al. (2013) Trends Immunol. 34:556.
Tamura, H. et al. (2001) Blood 97:1809.
Chen, L. et al. (2007) J. Immunol. 178:6634.
Kuang, D.-M. et al. (2014) J. Clin. Invest. 124:4657.
Tsushima, F. et al. (2007) Blood 110:180.
Mazanet, M.M. and C.C.W. Hughes (2002) J. Immunol. 169:3581.
Cao, Y. et al. (2010) Cancer Res. 71:1235.
Scandiuzzi, L. et al. (2014) Cell Rep. 6:625.
Dong, H. et al. (2002) Nat. Med. 8:793.
Azuma, T. et al. (2008) Blood 111:3635.
Butte, M.J. et al. (2008) Mol. Immunol. 45:3567.
Park, J.-J. et al. (2010) Blood 116:1291.
Ritprajak, P. et al. (2010) J. Immunol. 184:4918.
Herold, M. et al. (2015) J. Immunol. 195:3584.
Long Name:
B7 Homolog 1
Entrez Gene IDs:
29126 (Human); 60533 (Mouse); 499342 (Rat); 484186 (Canine); 102145573 (Cynomolgus Monkey)
Alternate Names:
B7-H; B7H1; B7-H1; B7H1PDCD1L1; CD274 antigenMGC142294; CD274 molecule; CD274; PDCD1L1; PDCD1LG1; PDCD1LG1MGC142296; PDL1; PD-L1; PD-L1B7 homolog 1; PDL1PDCD1 ligand 1; programmed cell death 1 ligand 1; Programmed death ligand 1
