Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured in a competitive binding assay. When rmICOS/Fc Chimera (Catalog # 168-CS) is immobilized at 1 µg/mL (100 µL/well), rmB7-H2 inhibits 50% binding of biotinylated rhB7-H2/Fc Chimera (2.5 ng/mL) at the concentration range of 80‑120 ng/mL.
Source
Mouse myeloma cell line, NS0-derived
Mouse B7-H2
(Glu47 - Lys279)
Accession # Q9JHJ8DIEGRMD Human IgG1
(Pro100 - Lys330)N-terminus C-terminus Accession #
N-terminal Sequence
AnalysisGlu47
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
53 kDa (monomer)
SDS-PAGE
80-90 kDa, reducing conditions
158-B7 |
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Formulation Lyophilized from a 0.2 μm filtered solution in PBS. | ||
Reconstitution Reconstitute at 100 μg/mL in sterile PBS. | ||
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | ||
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Background: B7-H2
Mouse B7-H2, also called B7RP-1, B7h, LICOS, and GL50, is a member of the growing B7 family of immune co‑stimulatory proteins. Other family members include B7-1, B7-2, B7-H1 (PD-L1), PD-L2, and B7-H3. B7 proteins are members of the immunoglobulin (Ig) superfamily. The extracellular domains contain 2 Ig-like domains and all members have short cytoplasmic domains. Among the family members, they share about 20 - 25% amino acid identity. Mouse and human B7-H2 share approximately 49% amino acid identity. B7-H2 has been identified as the ligand for ICOS, a member of the CD28 family of co‑stimulatory receptors. Mouse B7-H2 is a 322 amino acid (aa) protein with a putative 46 aa signal peptide, a 233 aa extracellular domain, a 19 aa transmembrane region, and a 24 aa cytoplasmic domain. Mouse B7-H2 is expressed constitutively on resting B cells and at low levels on monocytes. The B7-H2/ICOS interaction appears to play roles in T cell dependent B cell activation and Th differentiation.
References:
Coyle, A.J. and J.C. Gutierrez-Ramos (2001) Nat. Immunol. 2:203.
Ling, V. et al. (2000) J. Immunol. 164:1653.
Wang, S. et al. (2000) Blood 96:2808.
Brodie, D. et al. (2000) Curr. Biol. 10:333.
Mages, H.W. et al. (2000) Eur. J. Immunol. 30:1040.
Swallow, M.M. et al. (1999) Immunity 11:423.
Yoshinaga, S.K. et al. (1999) Nature 402:827.
Long Name:
B7 Homolog 2
Entrez Gene IDs:
23308 (Human); 50723 (Mouse); 102132485 (Cynomolgus Monkey)
Alternate Names:
B7H2; B7-H2; B7H2B7 homolog 2; B7-H2B7-related protein 1; B7RP-1; B7RP1B7-like protein Gl50; B7RP-1LICOS; CD275 antigen; CD275; GL50; GL50transmembrane protein B7-H2 ICOS ligand; ICOSL; ICOS-L; ICOSLG; inducible T-cell co-stimulator ligand; KIAA0653ICOS ligand
