Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit IL-17-induced IL-6 secretion by NIH‑3T3 mouse embryonic fibroblast cells. The ED 50 for this effect is 0.015-0.075 µg/mL in the presence of 10 ng/mL of recombinant mouse IL-17.
Source
Mouse myeloma cell line, NS0-derived
Mouse IL-17 RA/IL-17 R
(Ser32-Trp322)
Accession # Q60943IEGRMDP Mouse IgG2A
(Glu98-Lys330)N-terminus C-terminus Accession #
N-terminal Sequence
AnalysisSer32
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
60.4 kDa (monomer)
SDS-PAGE
80-95 kDa, reducing conditions
4481-MR |
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Formulation Lyophilized from a 0.2 μm filtered solution in PBS. | ||
Reconstitution Reconstitute at 100 μg/mL in sterile PBS. | ||
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | ||
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Background: IL-17 RA/IL-17 R
IL-17 R, also known as IL-17 RA, is a 120 kDa type I transmembrane glycoprotein protein that plays a central role in inflammatory responses (1-3). Mature mouse IL‑17 R consists of a 291 amino acid (aa) extracellular domain, a 21 aa transmembrane segment, and a 521 aa cytoplasmic domain (4). The cytoplasmic domain contains a region homologous to the TIR domain of the TLR/IL-1 R family (5). Mouse IL-17 R shares 84% and 72% aa sequence identity with rat and human IL-17 R, respectively. Within the extracellular domain, it shares 18%-25 % sequence identity with mouse IL-17 RB, C, D, and E. While the expression of IL-17 is restricted to activated T cells, IL-17 R exhibits a broad tissue distribution (4). Even in the absence of ligand, IL-17 R exists on the cell surface as a multimer (6). IL-17 R can bind IL-17 but must associate with IL-17 RC to transduce signals (7, 8). Interestingly, human IL-17 R does not appear to form productive complexes with mouse IL-17 RC (8). The IL-17 R can also signal in response to IL-17F (9). IL-17 R ligation promotes T cell activation and the production of IL-6, G-CSF, SCF, and multiple pro‑inflammatory chemokines (4, 7, 9, 10). IL-17A and IL-17F synergize with TNF-alpha in the induction of CXCL1, G-CSF, and IL-6 (9, 11). This effect requires the presence of both TNF RI and TNF RII (9). IL-17 interactions with IL-17 R also inhibit the TNF-alpha induced up-regulation of fibroblast CCL5 and VCAM-1 (11). CCL5 and VCAM-1 induced effects are differentially sensitive to blockade with IL-17 R specific antibodies, suggesting that IL-17 R triggers divergent intracellular signals (11). In vivo, IL‑17 R activity is important for increased generation of neutrophils and their recruitment to sites of inflammation (10, 12, 13). IL-17 R is required for host defense against microbial infection and for the progression of arthritis from inflammation to destructive joint erosion (10, 13).
References:
Iwakura, Y. and H. Ishigame (2006) J. Clin. Invest. 116:1218.
Moseley, T.A. et al. (2003) Cytokine Growth Factor Rev. 14:155.
Kawaguchi, M. et al. (2004) J. Allergy Clin. Immunol. 114:1265.
Yao, Z. et al. (1995) Immunity 3:811.
Novatchkova, M. et al. (2003) Trends Biochem. Sci. 28:226.
Kramer, J.M. et al. (2006) J. Immunol. 176:711.
Hymowitz, S.G. et al. (2001) EMBO J. 20:5332.
Toy, D. et al. (2006) J. Immunol. 177:36.
McAllister, F. et al. (2005) J. Immunol. 175:404.
Ye, P. et al. (2001) J. Exp. Med. 194:519.
Schnyder, B. et al. (2005) Cytokine 31:191.
Tan, W. et al. (2006) J. Immunol. 176:6186.
Lubberts, E. et al. (2005) J. Immunol. 175:3360.
Long Name:
Interleukin 17 Receptor
Entrez Gene IDs:
23765 (Human); 16172 (Mouse); 312679 (Rat)
Alternate Names:
CD217 antigen; CD217; Cdw217; CDw217interleukin 17 receptor; hIL-17R; IL-17 R; IL-17 RA; IL-17 receptor A; IL17RA; IL-17RA; IL-17RAMGC10262; IL17Rinterleukin-17 receptor A; interleukin 17 receptor A
