Recombinant Mouse Osteoprotegerin/TNFRSF11B Fc Chimera, CF 100 UG

• Purity

  >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

• Endotoxin Level

  <0.10 EU per 1 μg of the protein by the LAL method.  

• Activity

  Measured by its ability to inhibit TRAIL-mediated cytotoxicity using L‑929 mouse fibroblast cells treated with TRAIL. The ED    ₅₀ for this effect is 3-15 ng/mL in the presence of 12 ng/mL of cross-linked Recombinant Human TRAIL/TNFSF10 (Catalog #s  and ).

• Source

  Mouse myeloma cell line, NS0-derived

  Mouse OPG Glu22-Leu401 (Gln138Arg) Accession # Q6PI12	IEGRMD	Human IgG1 (Pro100-Lys330) (Thr276Ala)	6-His tag
  N-terminus			C-terminus

• Accession #

• N-terminal Sequence    
  Analysis

  Glu22

• Structure / Form

  Disulfide-linked homodimer

• Predicted Molecular Mass

  70.9 kDa (monomer)

• SDS-PAGE

  85-95 kDa, reducing conditions

Background: Osteoprotegerin/TNFRSF11B

Osteoprotegerin (OPG), also called OCIF (osteoclastogenesis inhibitory factor) is a secreted 55-60 kDa protein that regulates bone density (1-3). As a member of the tumor necrosis factor receptor (TNFR) superfamily of proteins, it is designated TNFRSF11B (1-4). Mouse OPG cDNA encodes 401 amino acids (aa) including a 21 aa signal peptide and a 380 aa mature soluble protein with four TNFR domains, two death domains and a heparin‑binding region (4). The cysteine‑rich TNFR domains are essential for ligand interaction, while a cysteine at the C‑terminus mediates homodimerization (4). Mature mouse OPG shares 86%, 94%, 86%, 86% and 83% amino acid sequence identity with human, rat, equine, canine and bovine OPG, respectively. OPG is widely expressed and constitutively released as a homodimer by mesenchymal stem cells, fibroblasts and endothelial cells (1, 2, 5, 7). Regulation of its expression by estrogen, parathyroid hormone and cytokines is complex and changes with age (2). OPG has been called a decoy receptor for the TNF superfamily ligands, TRANCE (tumor necrosis factor‑related activation‑induced cytokine), also called RANK L (receptor activator of NF kappa B ligand), and TRAIL (TNF‑related apoptosis‑inducing ligand), which also bind TNF family receptors RANK and TRAIL receptors 1-4, respectively (2, 6). TRAIL decreases the release of OPG from cells that express it, while OPG inhibits TRAIL‑induced apoptosis (5, 6). Expression of RANK L on the cell surface, and thus its ability to stimulate osteoclastogenesis, is regulated by OPG by intracellular and extracellular mechanisms (7). Within osteoblasts, interaction of the basic domain of OPG with RANK L in the Golgi inhibits RANK L secretion (7). Extracellularly, OPG binding to RANK L results in clathrin‑mediated internalization and degradation of both proteins (7, 8). Binding of OPG by syndecan‑1 heparin sulfates on multiple myeloma cells also results in OPG internalization and degradation, contributing to bone loss (8, 9). OPG deficiency can cause juvenile Paget’s disease in humans, and insufficient OPG to balance with RANK L and RANK can produce osteoporosis and vascular calcification in both mice and humans (2, 10, 11).

• References:

1. Simonet, W.S. et al. (1997) Cell 89:309.

2. Trouvin, A-P. and V. Goeb 2010) Clin. Interv. Aging 5:345.

3. Yasuda, H. et al. (1998) Proc. Natl. Acad. Sci. USA 95:3597.

4. Yamaguchi, K. et al. (1998) J. Biol. Chem. 273:5117.

5. Corallini, F. et al. (2010) J. Cell. Physiol. Dec. 6 [Epub ahead of print].

6. Emery, J.G. et al. (1998) J. Biol. Chem. 273:14363.

7. Aoki, S. et al. (2010) J. Bone Miner. Res. 25:1907.

8. Tat, S.K. et al. (2006) Bone 39:706.

9. Standal, T. et al. (2002) Blood 100:3002.

10. Whyte, M.P. et al. (2002) N. Engl. J. Med. 347:175.

11. Van Campenhout, A. and J. Golledge (2009) Atherosclerosis 204:321.

• Entrez Gene IDs:

  4982 (Human); 18383 (Mouse)

• Alternate Names:

  OCIF; OCIFMGC29565; OPGtumor necrosis factor receptor superfamily member 11B; Osteoclastogenesis inhibitory factor; Osteoprotegerin; TNFRSF11B; TR1; tumor necrosis factor receptor superfamily, member 11b