Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit the IL-3-induced proliferation of NFS‑60 mouse myelogenous leukemia lymphoblast cells. The ED 50 for this effect is 0.2‑1 µg/mL in the presence of 2.5 µg/mL soluble rmIL-3 R alpha /Fc Chimera and 0.5 ng/mL of rmIL-3..
Source
Mouse myeloma cell line, NS0-derived
Mouse IL-3 R beta
(His23 - Trp440)
Accession # Q3U3P4IEGRID Human IgG1
(Pro100 - Lys330)N-terminus C-terminus Accession #
N-terminal Sequence
AnalysisHis23
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
74.9 kDa (monomer)
SDS-PAGE
80 kDa, reducing conditions
549-R3 |
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Formulation Lyophilized from a 0.2 μm filtered solution in PBS. | ||
Reconstitution Reconstitute at 100 μg/mL in sterile PBS. | ||
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | ||
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Background: IL-3 R beta
Interleukin 3 (IL-3) is a pleiotropic cytokine produced primarily by activated T cells or mast cells. IL-3 stimulates the proliferation and differentiation of hemopoietic cells including the pluripotent hematopoietic stem cells as well as various lineage-committed cells. The biological effects of IL-3 on the various cell types are mediated by the binding of IL-3 to specific cell surface receptor complexes. The functional high-affinity IL-3 receptor is a heterodimer consisting of a ligand binding alpha subunit and the beta subunit. The alpha subunit alone binds IL-3 with low affinity. The beta subunit is required for the high-affinity binding of IL-3 to the heterodimeric receptor complex. The beta subunit has also been found to be a component of the high-affinity receptor complex for IL-5 and GM-CSF and is also referred to as the beta common ( beta c) chain. In the mouse, there are two IL-3 R beta proteins. The first identified mouse IL-3 R beta was also called AIC2A and binds IL-3 with low affinity (1). The second mIL-3 R beta was referred to as AIC2B (2). AIC2B doesn’t bind IL-3 and is the homolog of the human IL-3 R beta. AIC2A was found to be the result of a gene duplication event. Both the alpha and the beta subunits are members of the cytokine receptor superfamily (3).
References:
Itoh, N. et al. (1990) Science 247:324.
Gorman, D.M. et al. (1990) Proc. Natl. Acad. Sci. USA 87:5459.
Schrader, J.W. in Cytokine Reference, (2001) J.J. Oppenheim and M. Feldmann, eds. Academic Press, New York, p. 1899.
Long Name:
Interleukin 3 Receptor beta
Entrez Gene IDs:
12984 (Mouse)
Alternate Names:
AIC2A; BetaIl3; Bil3; Csfgmrb; IL-3 R beta; IL3R beta; IL3R; IL3RB; IL-3Rb; Il3rb2
