Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by the ability of the immobilized protein to support the adhesion of LS180 human colorectal adenocarcinoma cells.
When 5 x 104 cells/well are added to Recombinant Mouse L‑Selectin/CD62L Fc Chimera coated plates, adhesion is induced in a dose dependent manner after a 1 hour incubation at 37 °C. The ED50 for this effect is 0.3‑1.2 μg/mL.
Optimal dilutions should be determined by each laboratory for each application.
Source
Mouse myeloma cell line, NS0-derived
Mouse L-Selectin
(Trp39 - Asn332)
Accession # Q3UV83IEGRMD Human IgG1
(Pro100 - Lys330)6-His tag N-terminus C-terminus Accession #
N-terminal Sequence
AnalysisTrp39
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
60.5 kDa (monomer)
SDS-PAGE
150 kDa, reducing conditions
576-LS |
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Formulation Lyophilized from a 0.2 μm filtered solution in PBS. | ||
Reconstitution Reconstitute at 0.1 mg/mL in sterile PBS. | ||
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | ||
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Background: L-Selectin/CD62L
L-Selectin (also known as Leukocyte Selectin, LAM‑1, LECAM-1, LECCAM-1, TQ1, Leu-8, MEL-14 antigen, DREG, lymph node homing receptor, and CD62L), a member of the Selectin family, is a cell surface glycoprotein expressed constitutively on a wide variety of leukocytes. Two forms of L-Selectin have been reported, apparently arising as a result of post-translational modifications. The lymphocyte form shows an apparent molecular weight of 74 kDa, while the neutrophil form is 90 - 100 kDa. Human and mouse L-Selectin share 76% amino acid sequence homology.
L-Selectin plays a role in the migration of lymphocytes into peripheral lymph nodes and sites of chronic inflammation, and of neutrophils into acute inflammatory sites. Acting in cooperation with P-Selectin and E-Selectin, L-Selectin mediates the initial interaction of circulating leukocytes with endothelial cells that produces a characteristic "rolling" of the leukocytes on the endothelium. This initial interaction involving ICAM-1 and VCAM-1 leads eventually to extravasation of the white blood cell through the blood vessel wall into the extracellular matrix tissue.
ELISA techniques have shown that detectable levels of soluble L-Selectin are present in the biological fluids of apparently normal individuals. Furthermore, a number of studies have reported that levels of L‑Selectin may be elevated or lowered in subjects with a variety of pathological conditions.
Entrez Gene IDs:
6402 (Human); 20343 (Mouse); 29259 (Rat)
Alternate Names:
CD62L antigen; CD62L; gp90-MEL; hLHRc; LAM1; LAM-1; LAM1LECAM1; LECAM1; LEU8; Leu-8; Leukocyte adhesion molecule 1; Leukocyte surface antigen Leu-8; Leukocyte-endothelial cell adhesion molecule 1; LNHR; LNHRTQ1; LSEL; L-Selectin; LYAM1; Lyam-1; LYAM1CD62 antigen-like family member L; Lymph node homing receptor; lymphocyte adhesion molecule 1; pln homing receptor; PLNHR; selectin L; SELL; TQ1
