Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit IL-21-dependent proliferation of N1186 human T cells Parrish-Novak, J. et al. (2000) Nature 408:57. The ED 50 for this effect is 0.75‑3 µg/mL in the presence of 100 ng/mL of recombinant mouse IL-21.
Source
Mouse myeloma cell line, NS0-derived
Mouse IL-21 R Subunit
(Cys20 - Pro236)
Accession # Q9JHX3IEGRMD Human IgG1
(Pro100 - Lys330)N-terminus C-terminus Accession #
N-terminal Sequence
AnalysisCys20
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
51.5 kDa (monomer)
SDS-PAGE
70-80 kDa, reducing conditions
596-MR |
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Formulation Lyophilized from a 0.2 μm filtered solution in PBS. | ||
Reconstitution Reconstitute at 100 μg/mL in sterile PBS. | ||
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | ||
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Background: IL-21 R
IL-21 R (interleukin-21 receptor) is a type I transmembrane glycoprotein within the class I cytokine receptor family, type 4 subfamily (1 ‑ 5). Complex formation between IL-21 R and the common gamma chain ( gamma c), also used for IL-2, IL-4, IL-7, IL-9, IL-13 and IL-15 receptors, is required for signaling (6, 7). Mouse IL-21 R cDNA encodes 521 amino acid (aa) including a 19 aa signal peptide, a 218 aa extracellular domain (ECD) with 4 conserved cysteine residues, a fibronectin type III domain, and a WSXWS motif, a 21 aa transmembrane domain and a 271 aa cytoplasmic domain with a Box 1 motif, a kinase domain, and several sites for tyrosine phosphorylation (4, 5). One such site, pY510, mediates STAT binding (1, 2). The mouse IL‑21 R ECD shares 69%, 91%, 65%, 63% and 58% aa identity with human, rat, equine, canine and bovine IL-21 R, respectively. One potential 447 aa isoform, with an alternate start site at aa 83, lacks the four conserved ECD cysteines. IL-21 R is expressed mainly on B cells (highest on mature, activated, follicular and germinal center B cells), NK cells, and activated T cells, but is also found on dendritic cells, alternatively activated macrophages, intestinal lamina propria fibroblasts and epithelial cells, and keratinocytes (1, 3 ‑ 5). Both IL‑21 and IL‑4 are necessary for efficient B cell IgG1 production and normal germinal center architecture (8). B cell IL‑21 R engagement induces Blimp-1 (which mediates plasma cell differentiation), and is important for memory responses (1, 9, 10). IL-21 R engagement on mouse NK cells enhances their cytotoxic activity and IFN-gamma production (4, 11). IL‑21 R engagement on CD8+ T cells aids control of viral infection and tumor growth; IL-21 R is also necessary for sufficient numbers of regulatory T cells to combat chronic inflammation (1, 12, 13). IL‑21 R expression is often up‑regulated in allergic skin inflammation, systemic lupus erythematosus and diffuse large B cell lymphoma (DLBCL) (1, 2, 14, 15).
References:
Leonard, W.J. et al. (2008) J. Leukoc. Biol. 84:348.
Konforte, D. et al. (2009) J. Immunol. 182:1791.
Monteleone, G. et al., 2009, Cytokine Growth Factor Rev. 20:185.
Parrish-Novak, et al. (2000) Nature 408:57.
Ozaki, K. et al. (2000) Proc. Natl. Acad. Sci. USA 97:11439.
Asao, H. et al. (2001) J. Immunol. 167:1.
Habib, T. et al. (2002) Biochemistry 41:8725.
Ozaki, K. et al. (2002) Science 298:1630.
Rankin, A.L. et al. (2011) J. Immunol. 186:667.
King, I.L. et al. (2010) J. Immunol. 185:6138.
Kasaian, M.T. et al. (2002) Immunity 16:559.
Frohlich, A. et al. (2009 Science 324:1576.
Tortola, L. et al. (2010) Blood 116:5200.
Jin, H. et al. (2009) J. Clin. Invest. 119:47.
Sarosiek, K.A. et al. (2010) Blood 115:570.
Long Name:
Interleukin 21 Receptor
Entrez Gene IDs:
50615 (Human); 60504 (Mouse)
Alternate Names:
CD360 antigen; CD360; IL-21 R; IL-21 receptor; IL21R; IL-21R; interleukin 21 receptor; interleukin-21 receptor; MGC10967; NILR; Novel interleukin receptor
