Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.1 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Mouse OSM R beta Fc Chimera is immobilized at 0.5 µg/mL and Recombinant Mouse gp130 Fc Chimera (Catalog # ) at 0.5 µg/mL (100 µL/well), the concentration of Recombinant Mouse Oncostatin M/OSM (Catalog # ) that produces 50% of the optimal binding response is approximately 0.2‑1 ng/mL.
Source
Mouse myeloma cell line, NS0-derived
Mouse OSM R beta
(Glu24-Leu738)
Accession # O70458IEGRMD Human IgG1
(Pro100-Lys330)6-His tag N-terminus C-terminus
Accession #
N-terminal Sequence
AnalysisGlu24
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
109 kDa (monomer)
SDS-PAGE
120-150 kDa, reducing conditions
662-OR |
| |
Formulation Lyophilized from a 0.2 μm filtered solution in PBS. | ||
Reconstitution Reconstitute at 100 μg/mL in sterile PBS. | ||
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | ||
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: OSM R beta
Oncostatin M (OSM) is a member of the IL-6 family of cytokines that share the gp130 as a common signal transducing receptor subunit. Human OSM signals through two types of human OSM receptor complexes: the type I complex comprising the leukemia inhibitory factor receptor beta (LIF R beta ) and gp130, the type II complex made up of OSM receptor beta (OSM R beta ) and gp130. In contrast, mouse OSM signals only through the mouse OSM R beta and gp130 complex. Mouse OSM R beta cDNA encodes a 971 amino acid (aa) residue type I transmembrane protein which contains a 23 aa residue signal peptide, an extracellular domain of 714 aa, a transmembrane domain of 20 aa and a 214 aa cytoplasmic domain. Mouse OSM R beta alone binds mOSM with low-affinity, but forms a high-affinity binding complex in the presence of gp130. Mouse OSM R beta is 55% identical at the amino acid sequence level to human OSM R beta.
References:
Lindberg, R.A. et al. (1998) Mol. Cell. Biol. 18:3357.
Tanaka, M. et al. (1999) Blood 93:804.
Long Name:
Oncostatin M Receptor beta
Entrez Gene IDs:
9180 (Human); 18414 (Mouse); 310132 (Rat)
Alternate Names:
OSM R beta; OSMR; OSMRB
