Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Bioassay data are not available.
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived
Mouse TROY
(Glu30-Leu170)
Accession # Q9JLL3DIEGRMD Human IgG1
(Pro100-Lys330)N-terminus C-terminus Accession #
N-terminal Sequence
AnalysisGlu30
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
42.3 kDa (monomer)
SDS-PAGE
45-50 kDa, reducing conditions
723-TR |
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Formulation Lyophilized from a 0.2 μm filtered solution in PBS. | ||
Reconstitution Reconstitute at 100 μg/mL in sterile PBS. | ||
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | ||
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Background: TROY/TNFRSF19
TROY (also known as TAJ and TNFRSF19) is a 45‑55 kDa member of the TNF receptor superfamily of molecules (1‑4). It is widely expressed in the embryo, but shows limited expression in adult. In particular, embryonicis found in embryonic epithelium, neural tube, and mesenchyme, and developing neurons of the hippocampus and thalamus (5, 6). In the adult,is found in the nervous system associated with dorsal root and retinal ganglia neurons, astrocytes, and microglia, and in hair follicles where it is expressed by dermal papilla matrix cells (4, 7, 8, 9). In human,has also been found on melanoma cells, not normal melanocytes (10). is reportedly a component of the Nogo-A receptor, taking the place of p75 in a membrane-bound LINGO-1:NgR1:complex (6, 8, 9). This complex purportedly mediates axon repulsion initiated by one of five myelin-associated inhibitory factors (OMpg; CD100; Ephrin B3; Nogo-A; MAG) (6, 11). In the hair follicle,binds LTa and acts with Eda to drive secondary hair follicle development (4, 12). Mature humanis a 394 amino acid (aa) type I transmembrane protein (1-3). It possesses a 141 aa extracellular region (aa 30-170) that contains three 40 aa TNFR cysteine motifs, and an extended 232 aa cytoplasmic domain (aa 192-423). There is one potential isoform that shows a two aa substitution for aa 416-423. The extracellular domains of human and mouseshare 90% aa identity.
References:
Hu, S. et al. (1999) Genomics 62:103.
Eby, M.T. et al. (2000) J. Biol. Chem. 275:15336.
Kojima, T. et al. (2000) J. Biol. Chem. 275:20742.
Hashimoto, T. et al. (2008) Cell Cycle 7:106.
Pispa, J. et al. (2003) Gene Exp. Patterns 3:675.
Mi, S. (2008) Cytokine Growth Factor Rev. 19:245.
Satoh, J. et al. (2007) Neuropathol. Appl. Neurobiol. 33:99.
Shao, Z. et al. (2005) Neuron 45:353.
Park, J.B. et al. (2000) Neuron 45:345.
Spanjaard, R.A. et al. (2007) Int. J. Cancer 120:1304.
Venkatesh, K. et al. (2007) J. Cell Biol. 177:393.
Pispa, J. et al. (2008) Hum. Mol. Genet. 17:3380.
Long Name:
Tumor Necrosis Factor Receptor Superfamily, Member 19
Entrez Gene IDs:
55504 (Human); 29820 (Mouse)
Alternate Names:
TAJ; TAJ-alpha; TAJToxicity and JNK inducer; TNFRSF19; TRADE; TROY; TROYtumor necrosis factor receptor superfamily member 19; tumor necrosis factor receptor superfamily, member 19
