Purity
>90%, by SDS-PAGE with silver staining
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit proliferation of PC‑3 human prostate cancer cells. The ED 50 for this effect is 5-30 ng/mL.
Source
Mouse myeloma cell line, NS0-derived
Mouse Ephrin-A2
(Glu23-Asn184)
Accession # P52801IEGRMDP Mouse IgG2A
(Glu98-Lys330)N-terminus C-terminus Accession #
N-terminal Sequence
AnalysisGlu23
Structure / Form
Disulfied-linked homodimer
Predicted Molecular Mass
46 kDa
SDS-PAGE
52-61 kDa, reducing conditions
8415-A2 |
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Formulation Lyophilized from a 0.2 μm filtered solution in PBS. | ||
Reconstitution Reconstitute at 250 μg/mL in sterile PBS. | ||
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | ||
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Background: Ephrin-A2
Ephrin-A2, also known as ELF-1, HEK7-L, LERK-6, and EPLG6, is an approximately 20 kDa member of the Ephrin-A family of GPI-anchored ligands that bind and induce the tyrosine autophosphorylation of Eph receptors. Ephrin-A ligands are structurally related to the extracellular domains of the transmembrane Ephrin-B ligands. Eph-Ephrin interactions are widely involved in the regulation of cell migration, tissue morphogenesis, and cancer progression. Ephrin-A2 preferentially interacts with receptors in the EphA family (1, 2). Mouse Ephrin-A2 is synthesized with a 20 amino acid (aa) signal peptide, a 164 aa mature chain, and a 25 aa C-terminal propeptide which is removed prior to GPI linkage of Ephrin-A2 to the membrane (3, 4). It shares 93% and 100% aa sequence identity with human and rat Ephrin-A2, respectively. Ephrin-A2 is expressed in discrete regions of the developing nervous system and limb buds (4-7). Its distribution complements the pattern of Eph receptor expression, and this plays an important role in tissue morphogenesis (7-9). Ephrin-A2 exerts an axon repulsive signal which is important for the accurate pathfinding of retinal ganglion cell axons to the tectum and hippocampal axons to the lateral septum (8, 10). Its up-regulation in astrocytes at sites of optic nerve damage may prevent re-innervation by retinal ganglion cells (11). Ephrin-A2 is also expressed on neural progenitor cells in the subventricular zone (SVZ). It interacts with EphA7, triggering reverse signaling through Ephrin-A2 and inhibition of progenitor cell proliferation (9). In the developing limbs, Ephrin-A2 regulates cartilage morphogenesis and the projection of motoneuron axons (6, 7, 12).
References:
Miao, H. and B. Wang (2009) Int. J. Biochem. Cell Biol. 41:762.
Pasquale, E.B. (2010) Nat. Rev. Cancer 10:165.
Shao, H. et al. (1995) J. Biol. Chem. 270:3467.
Cheng, H.-J., and J.G. Flanagan (1994) Cell 79:157.
Kenmuir, C.L. et al. (2012) Anat. Rec. (Hoboken) 295:105.
Ohta, K. et al. (1997) Mech. Dev. 64:127.
Wada, N. et al. (2003) Dev. Biol. 264:550.
Gao, P.-P. et al. (1996) Proc. Natl. Acad. Sci. USA 93:11161.
Holmberg, J. et al. (2005) Genes Dev. 19:462.
Nakamoto, M. et al. (1996) Cell 86:755.
Symonds, A.C.E. et al. (2007) Eur. J. Neurosci. 25:744.
Eberhart, J. et al. (2000) Dev. Neurosci. 22:237.
Entrez Gene IDs:
1943 (Human); 13637 (Mouse)
Alternate Names:
Cek7-L; EFNA2; ELF-1; EPH-related receptor tyrosine kinase ligand 6; EphrinA2; Ephrin-A2; EPLG6HEK7 ligand; HEK7-L; HEK7-ligand; LERK6; LERK6LERK-6; ligand of eph-related kinase 6
