Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. Immobilized recombinant rat UNC5H2 Fc Chimera at 5 µg/mL (100 µL/well) can bind Recombinant Mouse Netrin-1 with a linear range of 3‑200 ng/mL.
Source
Mouse myeloma cell line, NS0-derived
Mouse Netrin-1
(Val22-Ala603)
Accession # AAC52971IEGR GGGSGGGSGGGS 10-His tag N-terminus C-terminus Accession #
N-terminal Sequence
AnalysisVal22
Predicted Molecular Mass
68.2 kDa
SDS-PAGE
83 kDa, reducing conditions
Carrier Free
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
1109-N1 |
| 1109-N1/CF |
Formulation Lyophilized from a 0.2 μm filtered solution in PBS and EDTA with BSA as a carrier protein. | Formulation Lyophilized from a 0.2 μm filtered solution in PBS and EDTA with Trehalose. | |
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. | Reconstitution Reconstitute at 100 μg/mL in sterile PBS. | |
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | |
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
| Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Data Images
SDS-PAGE
| 1 μg/lane of Recombinant Mouse Netrin-1 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a band at 83 kDa. |
Background: Netrin-1
Mouse Netrin-1 is a member of the laminin-related family of axon-guidance molecules, collectively referred to as Netrins ( netr is Sanskrit for “one who guides”). The molecule’s cDNA encodes a 603 amino acid (aa) protein precursor that has structural similarity to the N-terminal gamma ‑chain of laminin. It contains a globular domain, three EGF repeats, and a C-terminal heparin-binding domain. Mouse Netrin-1 shares 52% aa identity with mouse Netrin-3, and 98% and 87% aa identity with human and chicken Netrin-1, respectively. Cells reported to express Netrin-1 in the embryo include cells of the floor plate, ventricular zone of the spinal cord, the brain, the ganglionic eminence, and parts of the diencephalon. Netrins were first identified for promoting the outgrowth of commissural axons and are also involved in helping migrating cells and axonal growth cones navigate to their targets. Netrins can provide both attractive and repulsive cues to neurons, depending on the receptors present and cellular context. In the adult, Netrin-1 is likely involved in axon regeneration in peripheral nerves. Netrin-1 has also been shown to be expressed outside of the nervous system and to be involved in development of such tissues as the pancreas, lung, bowel, bone and mammary gland. In non-neural organogenesis, Netrin‑1 provides an adhesive rather than guidance function. The DCC (deleted in colorectal carcinoma), Neogenin, the UNC5 family of receptors, and the adenosine A2b receptors are proposed to be functional receptors for Netrin-1 (1‑7).
References:
Puschel, A. (1999) Mech. Dev. 83:65.
Hedgecock, E. and C. Norris (1997) Trends Genet. 13:251.
Kappler, J. et al. (2000) Biochem. Biophys. Res. Commun. 271:287.
Madison, R. et al. (2000) Exp. Neurology 161:563.
Srinivasan, K. et al. (2003) Dev. Cell 4:371.
Livesey, F.J. (1999) Cell Mol. Life Sci. 56:62.
Corset, V. et al. (2000) Nature 407:747.
Entrez Gene IDs:
9423 (Human); 18208 (Mouse); 396389 (Chicken)
Alternate Names:
netrin 1; Netrin1; Netrin-1; NTN1; NTN1Lnetrin 1, mouse, homolog of
