Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to compete with Frizzled-1 for binding to biotinylated Wnt-3a. The IC 50 value is 0.7-3.5 nM, under conditions in which Recombinant Mouse (rm) Frizzled‑1 Fc Chimera (Catalog # ) is present at 2.1 nM, and biotinylated rmWnt-3a concentration is 2.7 nM.
Source
Mouse myeloma cell line, NS0-derived Leu25-Cys295, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
AnalysisLeu25
Predicted Molecular Mass
31.6 kDa
SDS-PAGE
35-40 kDa, reducing conditions
Carrier Free
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
1169-FR |
| 1169-FR/CF |
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein. | Formulation Lyophilized from a 0.2 μm filtered solution in PBS. | |
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. | Reconstitution Reconstitute at 100 μg/mL in sterile PBS. | |
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | |
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
| Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: sFRP-2
Secreted Frizzled Related Protein-2 (sFRP-2) belongs to a family of Wnt-binding proteins with homology to the ligand-binding domain of the Frizzled transmembrane Wnt receptors. The sFRP proteins are approximately 30 - 35 kDa in size and contain an N-terminal Frizzled-like domain with 10 conserved cysteines and a Netrin-like C-terminal domain (1, 2). Mature mouse sFRP-2, also known as SARP-1, SDF-5, and FRP-2, shares 99% aa sequence identity with human and rat sFRP-2 (3, 4). sFRP-2 is widely expressed during embryogenesis and in the adult in tissues including the eye, heart, lung, colon, intestine, smooth muscle, pancreas, prostate, testis, kidney, brain, teeth and joints, craniofacial mesenchyme, and preadipocytes (3 - 6). Depending on the context, sFRP-2 can exert either positive or negative effects on Wnt signaling (7 - 9). It also inhibits BMP-induced effects [Alfaro 35646, Oshima 3160]. sFRP-2 can be incorporated into the extracellular matrix through interactions with Fibronectin and Integrin alpha 5 beta 1 (11). sFRP-2 plays a variety of roles during tissue morphogenesis including inhibition of the planar cell polarity pathway and myoblast and osteoblast differentiation (8, 10, 12, 13). sFRP-2 is also expressed in multiple myeloma and glioma in which it promotes tumorigenicity (10, 14). At physiological concentrations sFRP-2 enhances BMP-1 mediated proteolysis of Pro-Collagen I, whereas at higher concentrations it inhibits BMP-1 activity (15, 16). This difference is significant considering that sFRP‑2 is up‑regulated in fibrotic areas of the heart following myocardial infarction (15, 16). Elevated levels of sFRP-2 then promote the recovery of cardiac function by reducing collagen deposition, remodeling, and calcification and by promoting the engraftment of mesenchymal stem cells into the heart (8, 15).
References:
Bovolenta, P. et al. (2008) J. Cell Sci. 121:737.
van Amerongen, R. and R. Nusse (2009) Development 136:3205.
Rattner, A. et al. (1997) Proc. Natl. Acad. Sci. 94:2859.
Melkonyan, H.S. et al. (1997) Proc. Natl. Acad. Sci. 94:13636.
Hu, E. et al. (1998) Biochem. Biophys. Res. Commun. 247:287.
Leimeister, C. et al. (1998) Mech. Dev. 75:29.
Galli, L.M. et al. (2006) Dev. Dyn. 235:681.
Alfaro, M.P. et al. (2010) J. Biol. Chem. 285:35645.
von Marschall, Z. and L.W. Fisher (2010) Biochem. Biophys. Res. Commun. 400:299.
Oshima, T. et al. (2005) Blood 106:3160.
Lee, J.-L. et al. (2004) J. Biol. Chem. 279:14602.
Sugiyama, Y. et al. (2010) Dev. Biol. 338:193.
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Kobayashi, K. et al. (2009) Nat. Cell Biol. 11:46.
Long Name:
Secreted Frizzled Related Protein 2
Entrez Gene IDs:
6423 (Human); 20319 (Mouse)
Alternate Names:
FRP2; FRP-2secreted apoptosis related protein 1; SARP-1; SARP1sFRP-2; SDF-5; Secreted apoptosis-related protein 1; secreted frizzled-related protein 2; sFRP2; sFRP-2
