Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit neurite outgrowth of dissociated E13 chick embryonic dorsal root ganglia (DRG) neurons. Able to significantly inhibit neurite outgrowth when immobilized as a 3 µL droplet containing 200 ng on a nitrocellulose-coated microplate.
All experiments were done under carrier-free conditions.Source
Mouse myeloma cell line, NS0-derived Ile25-Ser420, with a C-terminal 10-His tag
Accession #
N-terminal Sequence
AnalysisIle25
Predicted Molecular Mass
45.7 kDa
SDS-PAGE
100-120 kDa, reducing conditions
1674-MG |
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Formulation Lyophilized from a 0.2 μm filtered solution in PBS and NaCl. | ||
Reconstitution Reconstitute at 100 μg/mL in sterile PBS. | ||
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | ||
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Background: OMgp
Oligodendrocyte myelin glycoprotein (OMgp or OMG), Nogo, and myelin-associated glycoprotein (MAG), are three myelin-derived axon outgrowth inhibitors that collapse axonal growth cones and inhibit neurite outgrowth (1 - 3). These three structurally distinct proteins contribute to the myelin-associated inhibitory activity that prevents axonal regeneration after injury of the adult central nervous system (CNS). Mouse OMgp cDNA encodes a 440 amino acid (aa) residue glycosylphosphatidylinositol (GPI)-anchored protein that has a 24 aa signal peptide, eight leucine-rich repeats (LRR) followed by five serine/threonine-rich repeats (4). OMgp has multiple potential N-glycosylation and O-glycosylation sites. Mouse and human OMgp share approximately 88% aa sequence identity. OMgp is expressed on the surface of oligodendrocytes and on large projection neurons, including Purkinje cells of the cerebellum, pyramidal cells of the hippocampus, motoneurons of the brainstem and anterior horn cells of the spinal cord (5). The neurite outgrowth inhibitory activities of all three myelin-derived proteins are mediated by binding to a common receptor complex consisting of the Nogo receptor (NgR) and the p75 neurotrophin receptor (NGFR) (2, 3). Besides its function in the inhibition of axonal growth, OMgp has also been implicated in the inhibition of proliferation. Although the transmembrane receptor that mediates the proliferation inhibition activity has not been identified, the LRR repeats of OMgp were shown to be essential for both the proliferation inhibition and neurite outgrowth inhibition activities (6).
References:
Kottis, V. et al. (2002) J. Neurochem. 82:1566.
Wang, K. et al. (2002) Nature 417:941.
Wang, K. et al. (2002) Nature 420:74.
Mikol, D.D. et al. (1993) Genomics 17:604.
Habib, A. et al. (1998) J. Neurochem. 70:1704.
Vourc’h, P. et al. (2003) J. Neurochem. 85:889.
Long Name:
Oligodendrocyte-Myelin glycoprotein
Entrez Gene IDs:
4974 (Human); 18377 (Mouse)
Alternate Names:
oligodendrocyte myelin glycoprotein; OMG; OMgp; OMGPoligodendrocyte-myelin glycoprotein
